50 mM [U-2H] TRIS, 50 mM arginine, 50 mM glutamic acid, 10 uM zinc chloride, 5 mM [U-2H] mercaptoethanol, 50 mM sodium chloride, 95% H2O/5% D2O
95% H2O/5% D2O
2
50 mM [U-2H] TRIS, 50 mM arginine, 50 mM glutamic acid, 10 uM zinc chloride, 5 mM [U-2H] mercaptoethanol, 50 mM sodium chloride, 25 mg/ml tobacco mosaic virus, 95% H2O/5% D2O
95% H2O/5% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
50mM
TRIS-1
[U-2H]
1
50mM
arginine-2
1
50mM
glutamic acid-3
1
10uM
zinc chloride-4
1
5mM
mercaptoethanol-5
[U-2H]
1
50mM
sodium chloride-6
1
50mM
TRIS-7
[U-2H]
2
50mM
arginine-8
2
50mM
glutamic acid-9
2
10uM
zinc chloride-10
2
5mM
mercaptoethanol-11
[U-2H]
2
50mM
sodium chloride-12
2
25mg/mL
tobacco mosaic virus-13
2
試料状態
イオン強度: 0.125 / pH: 6.75 / 圧: ambient / 温度: 290 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
600
1
Bruker DRX
Bruker
DRX
500
2
-
解析
NMR software
名称
開発者
分類
TopSpin
BrukerBiospin
解析
Analysis
CCPN
chemicalshiftassignment
Analysis
CCPN
データ解析
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
構造決定
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
NMR constraints
NOE constraints total: 858 / NOE intraresidue total count: 2 / NOE long range total count: 277 / NOE medium range total count: 132 / NOE sequential total count: 371
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 50 / 登録したコンフォーマーの数: 20 / Maximum upper distance constraint violation: 0.43 Å