- PDB-2lfh: Solution NMR Structure of the Helix-loop-Helix Domain of Human ID... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 2lfh
Title
Solution NMR Structure of the Helix-loop-Helix Domain of Human ID3 Protein, Northeast Structural Genomics Consortium Target HR3111A
Components
DNA-binding protein inhibitor ID-3
Keywords
DNA binding protein inhibitor / Structural Genomics / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-Biology / Protein Structure Initiative / Helix-loop-helix
Function / homology
Function and homology information
cellular response to leptomycin B / leptomycin B binding / : / notochord development / multicellular organism development / negative regulation of myoblast differentiation / NGF-stimulated transcription / metanephros development / transcription regulator inhibitor activity / odontogenesis ...cellular response to leptomycin B / leptomycin B binding / : / notochord development / multicellular organism development / negative regulation of myoblast differentiation / NGF-stimulated transcription / metanephros development / transcription regulator inhibitor activity / odontogenesis / muscle organ development / regulation of DNA replication / transcription factor binding / negative regulation of osteoblast differentiation / epithelial cell differentiation / central nervous system development / circadian regulation of gene expression / neuron differentiation / negative regulation of DNA-binding transcription factor activity / response to wounding / heart development / cell differentiation / protein dimerization activity / regulation of cell cycle / positive regulation of apoptotic process / protein domain specific binding / negative regulation of DNA-templated transcription / positive regulation of gene expression / negative regulation of transcription by RNA polymerase II / nucleus / cytoplasm Similarity search - Function
DNA-bindingproteininhibitorID-3 / Class B basic helix-loop-helix protein 25 / bHLHb25 / Helix-loop-helix protein HEIR-1 / ID-like ...Class B basic helix-loop-helix protein 25 / bHLHb25 / Helix-loop-helix protein HEIR-1 / ID-like protein inhibitor HLH 1R21 / Inhibitor of DNA binding 3
Mass: 7639.716 Da / Num. of mol.: 2 / Fragment: Helix-loop-helix motif domain residues 27-83 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: 1R21, BHLHB25, HEIR1, ID3 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)+ Magic / References: UniProt: Q02535
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C CT-HSQC aliphatic
1
3
1
3D HNCO
1
4
1
(4,3)DGFTCABCA(CO)NHN
1
5
1
(4,3)D GFT HNNCABCA
1
6
1
2D 1H-13C HSQC aromatic
1
7
1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
1
8
1
3DHN(CA)CO
1
9
1
3D (H)CCH-COSY aliphatic
1
10
1
3D (H)CCH-TOCSY aliphatic
1
11
1
3DHBHA(CO)NH
1
12
1
2D 1H-15N LR-HSQC for histidines
1
13
1
1D15NT1
1
14
1
1D15NT2
1
15
2
2D 1H-13C CT-HSQC methyl
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.9 mM [U-100% 13C; U-100% 15N] HR3111A, 100 mM sodium chloride, 10 mM DTT, 10 mM TRIS, 0.02 % sodium azide, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.5 mM [5% 13C; U-100% 15N] HR3111A, 100 mM sodium chloride, 10 mM DTT, 10 mM TRIS, 0.02 % sodium azide, 90% H2O/10% D2O