- PDB-2l8t: Staphylococcus aureus pathogenicity island 1 protein gp6, an inte... -
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基本情報
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データベース: PDB / ID: 2l8t
タイトル
Staphylococcus aureus pathogenicity island 1 protein gp6, an internal scaffold in size determination
要素
Transposon Tn557 toxic shock syndrome toxin-1
キーワード
STRUCTURAL PROTEIN / scaffold / bacteriophage / sapi
機能・相同性
GTP Cyclohydrolase I; Chain A, domain 1 - #40 / Pathogenicity island protein gp6, Staphylococcus / Pathogenicity island protein gp6 superfamily / Pathogenicity island protein gp6 in Staphylococcus / GTP Cyclohydrolase I; Chain A, domain 1 / Helix non-globular / Special / Hypothetical mobile element-associated protein
ジャーナル: J Mol Biol / 年: 2011 タイトル: The Staphylococcus aureus pathogenicity island 1 protein gp6 functions as an internal scaffold during capsid size determination. 著者: Altaira D Dearborn / Michael S Spilman / Priyadarshan K Damle / Jenny R Chang / Eric B Monroe / Jamil S Saad / Gail E Christie / Terje Dokland / 要旨: Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can ...Staphylococcus aureus pathogenicity island 1 (SaPI1) is a mobile genetic element that carries genes for several superantigen toxins. SaPI1 is normally stably integrated into the host genome but can become mobilized by "helper" bacteriophage 80α, leading to the packaging of SaPI1 genomes into phage-like transducing particles that are composed of structural proteins supplied by the helper phage but having smaller capsids. We show that the SaPI1-encoded protein gp6 is necessary for efficient formation of small capsids. The NMR structure of gp6 reveals a dimeric protein with a helix-loop-helix motif similar to that of bacteriophage scaffolding proteins. The gp6 dimer matches internal densities that bridge capsid subunits in cryo-electron microscopy reconstructions of SaPI1 procapsids, suggesting that gp6 acts as an internal scaffolding protein in capsid size determination.