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- PDB-2l2c: NMR Structure of mosquito odorant binding protein bound to MOP ph... -

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Basic information

Entry
Database: PDB / ID: 2l2c
TitleNMR Structure of mosquito odorant binding protein bound to MOP pheromone
ComponentsOdorant-binding protein
KeywordsTRANSPORT PROTEIN / pheromone binding protein
Function / homology
Function and homology information


odorant binding / sensory perception of smell / extracellular space / metal ion binding
Similarity search - Function
Pheromone/general odorant binding protein domain / Insect pheromone/odorant binding protein domains. / Pheromone/general odorant binding protein / PBP/GOBP family / Pheromone/general odorant binding protein superfamily / Recoverin; domain 1 / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Odorant-binding protein
Similarity search - Component
Biological speciesCulex quinquefasciatus (southern house mosquito)
MethodSOLUTION NMR / simulated annealing
AuthorsAmes, J.
CitationJournal: To be Published
Title: 1H, 13C, 15N resonance assignments for odorant protein 1
Authors: Ames, J.
History
DepositionAug 16, 2010Deposition site: BMRB / Processing site: RCSB
Revision 1.0Jul 27, 2011Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2024Group: Data collection / Database references / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Odorant-binding protein


Theoretical massNumber of molelcules
Total (without water)14,5311
Polymers14,5311
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / 30structures with the lowest energy
RepresentativeModel #1closest to the average

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Components

#1: Protein Odorant-binding protein / CquiOBP1


Mass: 14530.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Culex quinquefasciatus (southern house mosquito)
Gene: CpipJ_CPIJ007604 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8T6I2
Has protein modificationY
Sequence detailsRESIDUE 77 WAS INADVERTANTLY MUTATED TO ARG DURING THE CLONING OF THE EXPRESSION CONSTRUCT

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D 1H-15N HSQC
1213D CBCA(CO)NH
1313D C(CO)NH
1413D HNCO
1513D HN(CA)CB
1613D HBHA(CO)NH
1713D H(CCO)NH

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Sample preparation

DetailsContents: 0.5 mM [U-98% 13C; U-98% 15N] CquiOBP1-1, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
SampleConc.: 0.5 mM / Component: CquiOBP1-1 / Isotopic labeling: [U-98% 13C; U-98% 15N]
Sample conditionsIonic strength: 20 / pH: 7 / Pressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz

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Processing

NMR softwareName: NMRPipe
Developer: Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Classification: processing
RefinementMethod: simulated annealing / Software ordinal: 1
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 30 / Conformers submitted total number: 15

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