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Yorodumi- PDB-2l2c: NMR Structure of mosquito odorant binding protein bound to MOP ph... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2l2c | ||||||
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Title | NMR Structure of mosquito odorant binding protein bound to MOP pheromone | ||||||
Components | Odorant-binding protein | ||||||
Keywords | TRANSPORT PROTEIN / pheromone binding protein | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Culex quinquefasciatus (southern house mosquito) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Ames, J. | ||||||
Citation | Journal: To be Published Title: 1H, 13C, 15N resonance assignments for odorant protein 1 Authors: Ames, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l2c.cif.gz | 594.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l2c.ent.gz | 510.8 KB | Display | PDB format |
PDBx/mmJSON format | 2l2c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l2/2l2c ftp://data.pdbj.org/pub/pdb/validation_reports/l2/2l2c | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14530.682 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Culex quinquefasciatus (southern house mosquito) Gene: CpipJ_CPIJ007604 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8T6I2 |
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Sequence details | RESIDUE 77 WAS INADVERTAN |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.5 mM [U-98% 13C; U-98% 15N] CquiOBP1-1, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.5 mM / Component: CquiOBP1-1 / Isotopic labeling: [U-98% 13C; U-98% 15N] |
Sample conditions | Ionic strength: 20 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software | Name: NMRPipe Developer: Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax Classification: processing |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 15 |