Pre-mRNA-processingfactor40homologA / Formin-binding protein 3 / Formin-binding protein 11 / Huntingtin-interacting protein A / ...Formin-binding protein 3 / Formin-binding protein 11 / Huntingtin-interacting protein A / Huntingtin yeast partner A / Huntingtin-interacting protein 10 / HIP-10 / Fas ligand-associated factor 1 / Renal carcinoma antigen NY-REN-6
Mass: 8251.467 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Description: N-His6-Lyp-Thr-FF-C, His6 - HisTag, Lyp - lipoyl domain solublity tag, Thr - thrombin cleavage site, FF - FF domain Gene: HYPA, FBP11, PRPF40A, FLAF1, FNBP3, HIP10 / Production host: Escherichia coli (E. coli) / Strain (production host): C41 / References: UniProt: O75400
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
CPMG
1
2
1
CPMG
NMR details
Text: Atoms HG2-CG-HG3 in PRO 47 A model 6 have unusual geometry due to a bug in CS-Rosetta software.
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1-2 mM [U-13C; U-15N; U-2H] FF-1, 100 mM sodium chloride-2, 50 mM sodium acetate-3, 90% H2O/10% D2O
90% H2O/10% D2O
2
1-2 mM [U-100% 13C; U-100% 15N; U-50% 2H] FF-4, 100 mM sodium chloride-5, 50 mM sodium acetate-6, 100% D2O
100% D2O
3
1-2 mM [selective 13C; U-100% 15N] FF-7, 100 mM sodium chloride-8, 50 mM sodium acetate-9, 90% H2O/10% D2O
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