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- PDB-2kxq: Solution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif ... -

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Basic information

Entry
Database: PDB / ID: 2kxq
TitleSolution Structure of Smurf2 WW2 and WW3 bound to Smad7 PY motif containing peptide
Components
  • E3 ubiquitin-protein ligase SMURF2
  • Smad7 PY motif containing peptide
KeywordsPROTEIN BINDING / WW / PY motif / Smurf2 / TGF-beta / modular binding
Function / homology
Function and homology information


positive regulation of chondrocyte hypertrophy / negative regulation of T-helper 17 type immune response / negative regulation of chondrocyte proliferation / negative regulation of T cell cytokine production / heteromeric SMAD protein complex / regulation of ventricular cardiac muscle cell membrane depolarization / negative regulation of T-helper 17 cell differentiation / regulation of transforming growth factor beta receptor signaling pathway / positive regulation of cell-cell adhesion / response to laminar fluid shear stress ...positive regulation of chondrocyte hypertrophy / negative regulation of T-helper 17 type immune response / negative regulation of chondrocyte proliferation / negative regulation of T cell cytokine production / heteromeric SMAD protein complex / regulation of ventricular cardiac muscle cell membrane depolarization / negative regulation of T-helper 17 cell differentiation / regulation of transforming growth factor beta receptor signaling pathway / positive regulation of cell-cell adhesion / response to laminar fluid shear stress / positive regulation of trophoblast cell migration / regulation of epithelial to mesenchymal transition / activin receptor binding / negative regulation of transcription by competitive promoter binding / negative regulation of ubiquitin-protein transferase activity / Signaling by BMP / type I transforming growth factor beta receptor binding / SMAD protein signal transduction / negative regulation of activin receptor signaling pathway / adherens junction assembly / HECT-type E3 ubiquitin transferase / protein-containing complex localization / negative regulation of epithelial to mesenchymal transition / I-SMAD binding / negative regulation of ossification / transcription regulator inhibitor activity / Wnt signaling pathway, planar cell polarity pathway / ventricular cardiac muscle tissue morphogenesis / artery morphogenesis / ureteric bud development / ventricular septum morphogenesis / negative regulation of SMAD protein signal transduction / negative regulation of peptidyl-threonine phosphorylation / SMAD binding / negative regulation of peptidyl-serine phosphorylation / negative regulation of BMP signaling pathway / regulation of cardiac muscle contraction / ubiquitin-like ligase-substrate adaptor activity / anatomical structure morphogenesis / ubiquitin ligase complex / negative regulation of protein ubiquitination / cellular response to transforming growth factor beta stimulus / collagen binding / transforming growth factor beta receptor signaling pathway / Downregulation of TGF-beta receptor signaling / negative regulation of cell migration / Asymmetric localization of PCP proteins / cellular response to leukemia inhibitory factor / Degradation of AXIN / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / negative regulation of transforming growth factor beta receptor signaling pathway / Hedgehog 'on' state / Regulation of RUNX3 expression and activity / fibrillar center / beta-catenin binding / ubiquitin-protein transferase activity / transcription corepressor activity / UCH proteinases / Interferon gamma signaling / ubiquitin protein ligase activity / positive regulation of canonical Wnt signaling pathway / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Antigen processing: Ubiquitination & Proteasome degradation / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / cell differentiation / protein stabilization / Ub-specific processing proteases / protein ubiquitination / nuclear speck / membrane raft / negative regulation of DNA-templated transcription / centrosome / ubiquitin protein ligase binding / chromatin / regulation of transcription by RNA polymerase II / negative regulation of transcription by RNA polymerase II / protein-containing complex / nucleoplasm / identical protein binding / nucleus / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain ...MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain / Domain A in dwarfin family proteins / Ubiquitin Ligase Nedd4; Chain: W; - #10 / E3 ubiquitin-protein ligase, SMURF1 type / SMAD-like domain superfamily / Ubiquitin Ligase Nedd4; Chain: W; / : / HECT domain / HECT, E3 ligase catalytic domain / HECT-domain (ubiquitin-transferase) / HECT domain profile. / Domain Homologous to E6-AP Carboxyl Terminus with / C2 domain / Protein kinase C conserved region 2 (CalB) / SMAD/FHA domain superfamily / WW domain / WW/rsp5/WWP domain signature. / C2 domain / C2 domain profile. / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / Single Sheet / C2 domain superfamily / Mainly Beta
Similarity search - Domain/homology
Mothers against decapentaplegic homolog 7 / E3 ubiquitin-protein ligase SMURF2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR
AuthorsChong, A. / Lin, H. / Wrana, J. / Forman-Kay, J.D.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2010
Title: Coupling of tandem Smad ubiquitination regulatory factor (Smurf) WW domains modulates target specificity.
Authors: Chong, P.A. / Lin, H. / Wrana, J.L. / Forman-Kay, J.D.
History
DepositionMay 11, 2010Deposition site: BMRB / Processing site: RCSB
Revision 1.0Oct 13, 2010Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Jun 20, 2012Group: Database references
Revision 1.3May 1, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: E3 ubiquitin-protein ligase SMURF2
B: Smad7 PY motif containing peptide


Theoretical massNumber of molelcules
Total (without water)12,3042
Polymers12,3042
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / 200structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein E3 ubiquitin-protein ligase SMURF2 / hSMURF2 / SMAD ubiquitination regulatory factor 2 / SMAD-specific E3 ubiquitin-protein ligase 2


Mass: 10114.009 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SMURF2 / Plasmid: PGEX6P1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21DE3 cp / References: UniProt: Q9HAU4
#2: Protein/peptide Smad7 PY motif containing peptide


Mass: 2190.409 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: Smad7 / Plasmid: PGEX6P1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21DE3 cp / References: UniProt: O15105*PLUS
Sequence detailsPEPTIDE SEQUENCE ELESPPPPYSRYPMD CORRESPONDS TO RESIDUES 203-217 OF UNIPROT ENTRY O15105.

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1113D CBCA(CO)NH
1213D HN(CA)CB
1313D (H)CCH-TOCSY
1413D NC-NOESY (N and C simultaneous)
151HACAN
1613D CCC-TOCSY-NNH
171(HB)CB(CGCD)HD ARO
181HBCBCGCDCDHE ARO

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Sample preparation

DetailsContents: 1.2 mM [U-100% 13C; U-100% 15N] WW23, 1.2 mM [U-100% 13C; U-100% 15N] S7PY, 90% H2O/10% D2O
Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)ComponentIsotopic labelingSolution-ID
1.2 mMWW23[U-100% 13C; U-100% 15N]1
1.2 mMS7PY[U-100% 13C; U-100% 15N]1
Sample conditionsIonic strength: 235 / pH: 7.2 / Pressure: ambient / Temperature: 298 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian INOVAVarianINOVA5001
Varian INOVAVarianINOVA5002

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Processing

NMR softwareName: CNS / Version: 1.1 / Developer: Brunger, A.T. et al. / Classification: refinement
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 200 / Conformers submitted total number: 10

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