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Yorodumi- PDB-2kuq: Solution structure of the chimera of the PTB domain of SNT-2 and ... -
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-Basic information
Entry | Database: PDB / ID: 2kuq | ||||||
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Title | Solution structure of the chimera of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of HALK | ||||||
Components | Fibroblast growth factor receptor substrate 3,LINKER,ALK tyrosine kinase receptor | ||||||
Keywords | SIGNALING PROTEIN / Chimera / SNT-2 / PTB domain / hALK / Structural genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information ASP-3026-resistant ALK mutants / NVP-TAE684-resistant ALK mutants / alectinib-resistant ALK mutants / brigatinib-resistant ALK mutants / ceritinib-resistant ALK mutants / crizotinib-resistant ALK mutants / lorlatinib-resistant ALK mutants / MDK and PTN in ALK signaling / receptor signaling protein tyrosine kinase activator activity / regulation of dopamine receptor signaling pathway ...ASP-3026-resistant ALK mutants / NVP-TAE684-resistant ALK mutants / alectinib-resistant ALK mutants / brigatinib-resistant ALK mutants / ceritinib-resistant ALK mutants / crizotinib-resistant ALK mutants / lorlatinib-resistant ALK mutants / MDK and PTN in ALK signaling / receptor signaling protein tyrosine kinase activator activity / regulation of dopamine receptor signaling pathway / response to environmental enrichment / fibroblast growth factor receptor binding / ALK mutants bind TKIs / swimming behavior / transmembrane receptor protein tyrosine kinase adaptor activity / positive regulation of dendrite development / RND1 GTPase cycle / RND2 GTPase cycle / regulation of neuron differentiation / Signaling by ALK / adult behavior / neuron development / fibroblast growth factor receptor signaling pathway / Activated NTRK2 signals through FRS2 and FRS3 / negative regulation of lipid catabolic process / peptidyl-tyrosine autophosphorylation / FRS-mediated FGFR3 signaling / energy homeostasis / FRS-mediated FGFR2 signaling / FRS-mediated FGFR4 signaling / cell surface receptor protein tyrosine kinase signaling pathway / FRS-mediated FGFR1 signaling / transmembrane receptor protein tyrosine kinase activity / phosphorylation / hippocampus development / receptor protein-tyrosine kinase / Signaling by ALK fusions and activated point mutants / positive regulation of NF-kappaB transcription factor activity / heparin binding / regulation of cell population proliferation / RAF/MAP kinase cascade / protein tyrosine kinase activity / regulation of apoptotic process / protein autophosphorylation / receptor complex / signal transduction / protein-containing complex / extracellular exosome / ATP binding / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) synthetic construct (others) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Li, H. / Koshiba, S. / Tomizawa, T. / Watanabe, S. / Harada, T. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: J.Struct.Funct.Genom. / Year: 2010 Title: Structural basis for the recognition of nucleophosmin-anaplastic lymphoma kinase oncoprotein by the phosphotyrosine binding domain of Suc1-associated neurotrophic factor-induced tyrosine-phosphorylated target-2 Authors: Koshiba, S. / Li, H. / Motoda, Y. / Tomizawa, T. / Kasai, T. / Tochio, N. / Yabuki, T. / Harada, T. / Watanabe, S. / Tanaka, A. / Shirouzu, M. / Kigawa, T. / Yamamoto, T. / Yokoyama, S. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR DETERMINED |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kuq.cif.gz | 896.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kuq.ent.gz | 754.5 KB | Display | PDB format |
PDBx/mmJSON format | 2kuq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ku/2kuq ftp://data.pdbj.org/pub/pdb/validation_reports/ku/2kuq | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16953.816 Da / Num. of mol.: 1 / Fragment: PTB domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human), (gene. exp.) synthetic construct (others) Gene: FRS3, ALK / Production host: E. coli-cell-free protein synthesis / References: UniProt: O43559, UniProt: Q9UM73 |
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Sequence details | REISDUES 121-134 ARE LINKER |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.12mM [U-13C; U-15N] (chain A)-1, 20mM [U-2H] TRIS-2, 100mM sodium chloride-3, 1mM [U-2H] DTT-4, 0.02% sodium azide-5, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 120 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 / Representative conformer: 1 |