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Open data
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Basic information
Entry | Database: PDB / ID: 2kuk | ||||||
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Title | Solution structure of vhl-2 | ||||||
![]() | Leaf cyclotide 2 | ||||||
![]() | ANTIVIRAL PROTEIN / beta strands / beta turn / Disulfide bond / Knottin / Plant defense | ||||||
Function / homology | Cyclotide, moebius, conserved site / Cyclotides Moebius subfamily signature. / Cyclotides profile. / Cyclotide / Cyclotide superfamily / Cyclotide family / hemolysis in another organism / defense response / Leaf cyclotide 2![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Daly, N.L. / Chen, B. / Nguyencong, P. / Craik, D.J. | ||||||
![]() | ![]() Title: Structure and Activity of the Leaf-Specific Cyclotide vhl-2 Authors: Daly, N.L. / Chen, B. / Nguyencong, P. / Craik, D.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 158.7 KB | Display | ![]() |
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PDB format | ![]() | 137.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 338.2 KB | Display | ![]() |
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Full document | ![]() | 406 KB | Display | |
Data in XML | ![]() | 10.7 KB | Display | |
Data in CIF | ![]() | 17.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3200.624 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1 mM vhl-2-1, 75%H2O/5%D2O/25%ACN / Solvent system: 75%H2O/5%D2O/25%ACN |
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Sample | Conc.: 1 mM / Component: vhl-2-1 |
Sample conditions | Ionic strength: 0 / pH: 4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker ARX / Manufacturer: Bruker / Model: ARX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 / Representative conformer: 1 |