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Yorodumi- PDB-2kmw: Solution structure of At3g03773.1 protein from Arabidopsis thaliana -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kmw | |||||||||
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Title | Solution structure of At3g03773.1 protein from Arabidopsis thaliana | |||||||||
Components | Uncharacterized protein At3g03773 | |||||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Protein Structure Initiative / PSI-2 / Center for Eukaryotic Structural Genomics / CESG | |||||||||
Function / homology | Function and homology information protein folding chaperone complex / chaperone cofactor-dependent protein refolding / chaperone-mediated protein complex assembly / Hsp90 protein binding / protein folding / response to heat / protein-folding chaperone binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | Arabidopsis thaliana (thale cress) | |||||||||
Method | SOLUTION NMR / simulated annealing, distance geometry | |||||||||
Model details | lowest energy, model 1 | |||||||||
Authors | Sahu, S.C. / Singh, S. / Tonelli, M. / Markley, J.L. / Center for Eukaryotic Structural Genomics (CESG) | |||||||||
Citation | Journal: To be Published Title: Solution structure of At3g03773.1 protein from Arabidopsis thaliana Authors: Sahu, S.C. / Singh, S. / Tonelli, M. / Markley, J.L. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kmw.cif.gz | 927.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kmw.ent.gz | 782.9 KB | Display | PDB format |
PDBx/mmJSON format | 2kmw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/km/2kmw ftp://data.pdbj.org/pub/pdb/validation_reports/km/2kmw | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17342.174 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: At3g03773, F20H23.30 / Production host: Escherichia coli (E. coli) / References: UniProt: Q6ID70 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1 mM [U-13C; U-15N] protein, 93% H2O/7% D2O / Solvent system: 93% H2O/7% D2O |
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Sample | Conc.: 1 mM / Component: protein-1 / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 0.1 / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, distance geometry / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |