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- PDB-2kmi: MESD(12-155), The Core Structural Domain of MESD that Is Essentia... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2kmi | ||||||
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Title | MESD(12-155), The Core Structural Domain of MESD that Is Essential for Proper Folding of LRP5/6 | ||||||
![]() | Mesoderm development candidate 2 | ||||||
![]() | CHAPERONE / MESD/Boca / LRP5/6 | ||||||
Function / homology | ![]() positive regulation of skeletal muscle acetylcholine-gated channel clustering / protein localization to cell surface / low-density lipoprotein particle receptor binding / mesoderm development / phagocytosis / ossification / Wnt signaling pathway / protein folding / endoplasmic reticulum / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Chen, J. / Wang, J. | ||||||
![]() | ![]() Title: NMR structure note: solution structure of the core domain of MESD that is essential for proper folding of LRP5/6. Authors: Chen, J. / Li, Q. / Liu, C.C. / Zhou, B. / Bu, G. / Wang, J. #1: Journal: Cell(Cambridge,Mass.) / Year: 2003 Title: Coaxing the LDL receptor family into the fold Authors: Herz, J. / Marschang, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1 MB | Display | ![]() |
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PDB format | ![]() | 887 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 343.4 KB | Display | ![]() |
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Full document | ![]() | 494.7 KB | Display | |
Data in XML | ![]() | 53.4 KB | Display | |
Data in CIF | ![]() | 66.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 16692.848 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Mesoderm development (MESD) protein is a 195-residue protein that serves as a specialized molecular chaperone to promote the proper folding of the six-bladed ??-propeller/EGF modules of the ...Details: Mesoderm development (MESD) protein is a 195-residue protein that serves as a specialized molecular chaperone to promote the proper folding of the six-bladed ??-propeller/EGF modules of the LDL receptor family members, and Mesd(12-155) is the most conserved region of Mesd protein. | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1.0 mM [U-13C; U-15N] mesd12-155, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.0 mM / Component: mesd12-155-1 / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | pH: 7 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |