THE DIFFERENCES BETWEEN THE DEPOSITED SEQUENCE AND THE SEQUENCE OF UNP ENTRY P23685 ORIGINATE FROM ...THE DIFFERENCES BETWEEN THE DEPOSITED SEQUENCE AND THE SEQUENCE OF UNP ENTRY P23685 ORIGINATE FROM THE FACT THAT THERE ARE SEVERAL SPLICE FORMS. P23685 CONTAINS ALL EXONS (A,C,D,E AND F). THE DEPOSITED STRUCTURE CONTAINS ONLY EXONS B AND D (OFTEN ALSO TERMED AS NCX1.3).
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-15N HSQC
1
2
1
3D HN(CA)CB
1
3
1
3DCBCA(CO)NH
1
4
1
3D (H)CCH-TOCSY
1
5
1
3D HNHA
1
6
1
3D 1H-15N NOESY
1
7
1
3D 1H-13C NOESY aliphatic
1
8
1
3D 1H-13C NOESY aromatic
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試料調製
詳細
内容: 0.5 mM [U-100% 13C; U-100% 15N] Na+/Ca2+ exchanger, 20 mM Hepes, 20 mM b-mercaptoethanol, 10 mM CaCl2, 95% H2O/5% D2O 溶媒系: 95% H2O/5% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
0.5mM
Na+/Ca2+ exchanger-1
[U-100% 13C; U-100% 15N]
1
20mM
Hepes-2
1
20mM
b-mercaptoethanol-3
1
10mM
CaCl2-4
1
試料状態
イオン強度: 0.02 / pH: 7.0 / 圧: ambient / 温度: 306 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
800
1
Varian INOVA
Varian
INOVA
600
2
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解析
NMR software
名称
バージョン
開発者
分類
CYANA
2.1
Herrmann, Guntert, Wuthrich
構造決定
X-PLOR
Brunger
精密化
精密化
手法: torsion angle dynamics / ソフトェア番号: 1
NMR constraints
NOE constraints total: 2324 / NOE long range total count: 1031
代表構造
選択基準: closest to the average
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20