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Yorodumi- PDB-2kbg: Solution structure of the second Fibronectin type-III module of NCAM2 -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kbg | ||||||
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Title | Solution structure of the second Fibronectin type-III module of NCAM2 | ||||||
Components | Neural cell adhesion molecule 2 | ||||||
Keywords | CELL ADHESION / Cell adhesion molecule / fibronectin type III module / NCAM2 / beta-sheet sandwich / Cell membrane / Glycoprotein / Immunoglobulin domain / Membrane / Phosphoprotein / Transmembrane | ||||||
Function / homology | Function and homology information neuron cell-cell adhesion / axonal fasciculation / nuclear body / axon / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Rasmussen, K.K. / Teilum, K. / Kulahin, N. / Clausen, O. / Berezin, V. / Bock, E. / Walmod, P.S. / Poulsen, F.M. | ||||||
Citation | Journal: To be Published Title: Solution structure of the second Fibronectin type-III module of NCAM2 Authors: Rasmussen, K.K. / Teilum, K. / Kulahin, N. / Clausen, O. / Berezin, V. / Bock, E. / Walmod, P.S. / Poulsen, F.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kbg.cif.gz | 636.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kbg.ent.gz | 551.7 KB | Display | PDB format |
PDBx/mmJSON format | 2kbg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kb/2kbg ftp://data.pdbj.org/pub/pdb/validation_reports/kb/2kbg | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13120.731 Da / Num. of mol.: 1 / Fragment: Fibronectin type-III domain 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NCAM2 / Production host: Pichia pastoris (fungus) / Strain (production host): KM71H / References: UniProt: O15394 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1934 / NOE intraresidue total count: 346 / NOE long range total count: 951 / NOE medium range total count: 146 / NOE sequential total count: 491 / Protein phi angle constraints total count: 59 / Protein psi angle constraints total count: 59 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 3 |