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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2kb7 | ||||||
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タイトル | Hybrid solution and solid-state NMR structure of monomeric phospholamban in lipid bilayers | ||||||
![]() | Phospholamban | ||||||
![]() | MEMBRANE PROTEIN / phospholamban / PISEMA / hybrid method / lipid bilayers / topology | ||||||
機能・相同性 | ![]() negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of relaxation of muscle / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / negative regulation of calcium ion transmembrane transporter activity / acrosome assembly / negative regulation of calcium ion import ...negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of relaxation of muscle / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / negative regulation of calcium ion transmembrane transporter activity / acrosome assembly / negative regulation of calcium ion import / negative regulation of catalytic activity / ATPase inhibitor activity / cardiac muscle tissue development / regulation of cardiac muscle cell contraction / enzyme inhibitor activity / negative regulation of heart rate / muscle cell cellular homeostasis / regulation of calcium ion transport / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Notch signaling pathway / sarcoplasmic reticulum membrane / mitochondrial membrane / intracellular calcium ion homeostasis / ATPase binding / endoplasmic reticulum membrane / perinuclear region of cytoplasm / endoplasmic reticulum / protein homodimerization activity / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / 個体NMR / simulated annealing | ||||||
![]() | Traaseth, N.J. / Shi, L. / Verardi, R. / Veglia, G. | ||||||
![]() | ![]() タイトル: Structure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approach. 著者: Traaseth, N.J. / Shi, L. / Verardi, R. / Mullen, D.G. / Barany, G. / Veglia, G. #1: ジャーナル: J.Biomol.Nmr / 年: 2009 タイトル: A refinement protocol to determine structure, topology, and depth of insertion of membrane proteins using hybrid solution and solid-state NMR restraints. 著者: Shi, L. / Traaseth, N.J. / Verardi, R. / Cembran, A. / Gao, J. / Veglia, G. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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-検証レポート
文書・要旨 | ![]() | 341.1 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 460.1 KB | 表示 | |
XML形式データ | ![]() | 18.2 KB | 表示 | |
CIF形式データ | ![]() | 30.4 KB | 表示 | |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 6150.477 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
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NMR実験 |
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試料調製
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試料状態 | pH: 6.0 / 圧: ambient / Temperature units: K |
-データ収集
NMRスペクトロメーター |
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解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: Authors state that they define the z-axis to be parallel with the bilayer normal. Also, the origin (or in other words, when the z Cartesian coordinate is 0) is defined to be the center of the lipid bilayer. | ||||||||||||||||
代表構造 | 選択基準: fewest violations | ||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 |