- PDB-1n7l: Solution NMR structure of phospholamban in detergent micelles -
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基本情報
登録情報
データベース: PDB / ID: 1n7l
タイトル
Solution NMR structure of phospholamban in detergent micelles
要素
Cardiac phospholamban
キーワード
SIGNALING PROTEIN / helix-turn-helix
機能・相同性
機能・相同性情報
phospholamban complex / negative regulation of calcium ion binding / circadian sleep/wake cycle, sleep / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / negative regulation of calcium ion import into sarcoplasmic reticulum / regulation of relaxation of muscle / negative regulation of ATPase-coupled calcium transmembrane transporter activity / regulation of the force of heart contraction by cardiac conduction / negative regulation of calcium ion import / calcium ion-transporting ATPase complex ...phospholamban complex / negative regulation of calcium ion binding / circadian sleep/wake cycle, sleep / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / negative regulation of calcium ion import into sarcoplasmic reticulum / regulation of relaxation of muscle / negative regulation of ATPase-coupled calcium transmembrane transporter activity / regulation of the force of heart contraction by cardiac conduction / negative regulation of calcium ion import / calcium ion-transporting ATPase complex / acrosome assembly / ATPase inhibitor activity / regulation of cardiac muscle cell contraction / cardiac muscle tissue development / regulation of cardiac muscle contraction by calcium ion signaling / negative regulation of heart rate / muscle cell cellular homeostasis / locomotor rhythm / regulation of calcium ion transport / enzyme inhibitor activity / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Notch signaling pathway / sarcoplasmic reticulum membrane / mitochondrial membrane / visual learning / intracellular calcium ion homeostasis / ATPase binding / endoplasmic reticulum membrane / perinuclear region of cytoplasm / endoplasmic reticulum / protein homodimerization activity / identical protein binding 類似検索 - 分子機能
Phospholamban / Phospholamban / Phospholamban / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
内容: 1 mM phospholamban U-15N,13C; 20 mM phosphate buffer; 90% H2O, 10% D2O; pH=4.2; 600 mM DPC;
試料状態
pH: 4.2 / 圧: 1 atm / 温度: 323 K
結晶化
*PLUS
手法: other / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
800
1
Varian INOVA
Varian
INOVA
600
2
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解析
NMR software
名称
バージョン
開発者
分類
VNMR
Varian
collection
NMRPipe
FrankDelaglio
解析
NMRView
5
BruceA. Johnson
データ解析
XPLOR-NIH
2.0.4
G. MariusClore , JohnKuszewski, CharlesD. Schwieters, andNicoTjandra
構造決定
XPLOR-NIH
2.0.4
Clore
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1
代表構造
選択基準: fewest violations
NMRアンサンブル
コンフォーマー選択の基準: structures with acceptable covalent geometry,structures with the least restraint violations,structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20