macrophage elastase / negative regulation of endothelial cell-matrix adhesion via fibronectin / bronchiole development / positive regulation of epithelial cell proliferation involved in wound healing / elastin catabolic process / regulation of defense response to virus by host / positive regulation of type I interferon-mediated signaling pathway / wound healing, spreading of epidermal cells / negative regulation of type I interferon-mediated signaling pathway / lung alveolus development ...macrophage elastase / negative regulation of endothelial cell-matrix adhesion via fibronectin / bronchiole development / positive regulation of epithelial cell proliferation involved in wound healing / elastin catabolic process / regulation of defense response to virus by host / positive regulation of type I interferon-mediated signaling pathway / wound healing, spreading of epidermal cells / negative regulation of type I interferon-mediated signaling pathway / lung alveolus development / response to amyloid-beta / positive regulation of interferon-alpha production / Collagen degradation / collagen catabolic process / extracellular matrix disassembly / core promoter sequence-specific DNA binding / collagen binding / Degradation of the extracellular matrix / extracellular matrix organization / extracellular matrix / cellular response to virus / metalloendopeptidase activity / protein import into nucleus / endopeptidase activity / sequence-specific DNA binding / serine-type endopeptidase activity / calcium ion binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / proteolysis / extracellular space / zinc ion binding / extracellular region / nucleus / cytoplasm 類似検索 - 分子機能
Text: Chemical shift assignments were previously reported, so experiments to support the assignments will not be described.
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試料調製
詳細
Solution-ID
内容
溶媒系
1
700 uM [U-100% 13C; U-100% 15N] protein, 700 uM DSV, 93% H2O/7% D2O
93% H2O/7% D2O
2
400 uM [U-100% 13C; U-100% 15N] protein, 400 uM DSV, 100% D2O
100% D2O
3
550 uM [U-10% 13C] protein, 550 uM DSV, 100% D2O
100% D2O
4
400 uM [U-100% 15N] protein, 400 uM DSV, 93% H2O/7% D2O
93% H2O/7% D2O
5
400 uM [U-100% 15N] protein, 400 uM DSV, 93% H2O/7% D2O
93% H2O/7% D2O
6
250 uM [U-100% 13C; U-100% 15N] protein, 250 uM DSV, 93% H2O/7% D2O
93% H2O/7% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
700uM
protein
[U-100% 13C; U-100% 15N]
1
700uM
DSV
1
400uM
protein
[U-100% 13C; U-100% 15N]
2
400uM
DSV
2
550uM
protein
[U-10% 13C]
3
550uM
DSV
3
400uM
protein
[U-100% 15N]
4
400uM
DSV
4
400uM
protein
[U-100% 15N]
5
400uM
DSV
5
250uM
protein
[U-100% 13C; U-100% 15N]
6
250uM
DSV
6
試料状態
イオン強度: 160 / pH: 6 / 圧: ambient / 温度: 298 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DRX
Bruker
DRX
600
1
Bruker DRX
Bruker
DRX
800
2
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解析
NMR software
名称
開発者
分類
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
PIPP
Garrett
データ解析
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
構造決定
X-PLOR NIH
Schwieters, Kuszewski, TjandraandClore
精密化
精密化
手法: DGSA-distance geometry simulated annealing, simulated annealing ソフトェア番号: 1 詳細: for structures with NOE and angle restraints, to incorporate dipolar couplings
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the least restraint violations 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 17