- PDB-2jzh: structure of IIB domain of the mannose transporter of E. coli -
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Basic information
Entry
Database: PDB / ID: 2jzh
Title
structure of IIB domain of the mannose transporter of E. coli
Components
PTS system mannose-specific EIIAB component
Keywords
TRANSFERASE / Mannose specific PTS system IIAB / IIB domain / IIBMan phosphotransferase enzyme II / B component / Cytoplasm / Membrane / Phosphoprotein / Phosphotransferase system / Sugar transport / Transport
Function / homology
Function and homology information
protein-Npi-phosphohistidine-D-mannose phosphotransferase / mannose transmembrane transport / protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity / glucose import across plasma membrane / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / kinase activity / protein homodimerization activity / membrane / plasma membrane ...protein-Npi-phosphohistidine-D-mannose phosphotransferase / mannose transmembrane transport / protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity / glucose import across plasma membrane / phosphoenolpyruvate-dependent sugar phosphotransferase system / transmembrane transporter complex / kinase activity / protein homodimerization activity / membrane / plasma membrane / cytosol / cytoplasm Similarity search - Function
Mass: 19119.258 Da / Num. of mol.: 1 / Fragment: PTS EIIB type-4 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: manX, gptB, ptsL / Plasmid: pET32a modified / Production host: Escherichia coli (E. coli) References: UniProt: P69797, protein-Npi-phosphohistidine-sugar phosphotransferase
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D (H)CCH-TOCSY
1
2
1
3D (H)CCH-COSY
1
3
1
3D 1H-13C NOESY
1
4
2
2D 1H-15N HSQC
1
5
2
3D 1H-15N NOESY
1
6
2
3DCBCA(CO)NH
1
7
2
3DC(CO)NH
1
8
2
3D HN(CA)CB
1
9
2
3DHBHA(CO)NH
1
10
2
3DH(CCO)NH
NMR details
Text: Double and triple resonance 3D NMR experiments for assignments (HNCACB, CBCA(CO)NH, HBHA(CBCACO)NH, C(CCO)NH, H(CCO)NH, HCCH-COSY, HCCH-TOCSY). NOE-derived interproton distance restrants from ...Text: Double and triple resonance 3D NMR experiments for assignments (HNCACB, CBCA(CO)NH, HBHA(CBCACO)NH, C(CCO)NH, H(CCO)NH, HCCH-COSY, HCCH-TOCSY). NOE-derived interproton distance restrants from 3D 15N-, 13C-, 13C/15M-, 13C/13C, and 15N/15N-separated NOE spectra. Side chain rotamers from 3H heteronuclear couplings and short mixing time 3D 13C-separated NOE and 3D 15N-seaparated ROE spectra. RDCs obtained by taking difference in J couplings in aligned (phage pf1 and PEG/hexanol) and isotopic media.
NOE constraints total: 1467 / NOE intraresidue total count: 451 / NOE long range total count: 339 / NOE medium range total count: 270 / NOE sequential total count: 407 / Protein chi angle constraints total count: 108 / Protein other angle constraints total count: 53 / Protein phi angle constraints total count: 160 / Protein psi angle constraints total count: 157
NMR representative
Selection criteria: minimized average structure
NMR ensemble
Conformer selection criteria: all calculated structures / Conformers calculated total number: 130 / Conformers submitted total number: 1
NMR ensemble rms
Distance rms dev: 0.006 Å / Distance rms dev error: 0.001 Å
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