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Yorodumi- PDB-2jvf: Solution structure of M7, a computationally-designed artificial p... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2jvf | ||||||
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Title | Solution structure of M7, a computationally-designed artificial protein | ||||||
Components | de novo protein M7 | ||||||
Keywords | DE NOVO PROTEIN / tetrapeptide fragment-based protein design / artificial fold | ||||||
Function / homology | top7, de novo designed protein / top7, de novo designed protein / 2-Layer Sandwich / Alpha Beta Function and homology information | ||||||
Biological species | unidentified (others) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Stordeur, C. / Dalluege, R. / Birkenmeier, O. / Wienk, H. / Rudolph, R. / Lange, C. / Luecke, C. | ||||||
Citation | Journal: Proteins / Year: 2008 Title: The NMR solution structure of the artificial protein M7 matches the computationally designed model Authors: Stordeur, C. / Dalluege, R. / Birkenmeier, O. / Wienk, H. / Rudolph, R. / Lange, C. / Luecke, C. #1: Journal: Proteins / Year: 2007 Title: A tetrapeptide fragment-based design method results in highly stable artificial proteins. Authors: Dalluge, R. / Oschmann, J. / Birkenmeier, O. / Lucke, C. / Lilie, H. / Rudolph, R. / Lange, C. | ||||||
History |
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Remark 999 | Sequence This is a de novo protein. The first four residues (GSHM) are cloning artifacts. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jvf.cif.gz | 600.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jvf.ent.gz | 501.8 KB | Display | PDB format |
PDBx/mmJSON format | 2jvf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jvf_validation.pdf.gz | 405.2 KB | Display | wwPDB validaton report |
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Full document | 2jvf_full_validation.pdf.gz | 492.2 KB | Display | |
Data in XML | 2jvf_validation.xml.gz | 29.2 KB | Display | |
Data in CIF | 2jvf_validation.cif.gz | 50.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jv/2jvf ftp://data.pdbj.org/pub/pdb/validation_reports/jv/2jvf | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10837.450 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) unidentified (others) / Plasmid: pET-28a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 25 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |