+Open data
-Basic information
Entry | Database: PDB / ID: 2jsi | ||||||
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Title | 11-23 obestatin fragment in DPC/SDS micellar solution | ||||||
Components | Appetite-regulating hormone, ObestatinGhrelin | ||||||
Keywords | HORMONE / 11-23 obestatin fragment / micellar solution / DPC / SDS / Alternative splicing / Amidation / Lipoprotein / Secreted | ||||||
Function / homology | Function and homology information positive regulation of response to food / positive regulation of gastro-intestinal system smooth muscle contraction / ghrelin receptor binding / positive regulation of bone development / positive regulation of gastric mucosal blood circulation / negative regulation of locomotion / positive regulation of small intestinal transit / growth hormone-releasing hormone activity / negative regulation of circadian sleep/wake cycle, REM sleep / positive regulation of circadian sleep/wake cycle, non-REM sleep ...positive regulation of response to food / positive regulation of gastro-intestinal system smooth muscle contraction / ghrelin receptor binding / positive regulation of bone development / positive regulation of gastric mucosal blood circulation / negative regulation of locomotion / positive regulation of small intestinal transit / growth hormone-releasing hormone activity / negative regulation of circadian sleep/wake cycle, REM sleep / positive regulation of circadian sleep/wake cycle, non-REM sleep / regulation of response to food / regulation of gastric motility / Synthesis, secretion, and deacylation of Ghrelin / regulation of transmission of nerve impulse / gastric acid secretion / positive regulation of corticotropin secretion / positive regulation of cortisol secretion / positive regulation of feeding behavior / positive regulation of growth / positive regulation of growth rate / positive regulation of eating behavior / positive regulation of appetite / positive regulation of small intestine smooth muscle contraction / G alpha (q) signalling events / adult feeding behavior / positive regulation of growth hormone secretion / actin polymerization or depolymerization / positive regulation of synapse assembly / negative regulation of interleukin-1 beta production / positive regulation of vascular endothelial cell proliferation / regulation of postsynapse organization / postsynaptic modulation of chemical synaptic transmission / protein tyrosine kinase activator activity / positive regulation of sprouting angiogenesis / dendrite development / negative regulation of endothelial cell proliferation / positive regulation of insulin secretion involved in cellular response to glucose stimulus / negative regulation of interleukin-6 production / decidualization / negative regulation of tumor necrosis factor production / negative regulation of insulin secretion / response to electrical stimulus / synapse assembly / positive regulation of adipose tissue development / excitatory postsynaptic potential / response to hormone / G protein-coupled receptor binding / Schaffer collateral - CA1 synapse / visual learning / positive regulation of insulin secretion / hormone activity / negative regulation of inflammatory response / response to estrogen / positive regulation of cold-induced thermogenesis / regulation of cell population proliferation / positive regulation of cytosolic calcium ion concentration / postsynapse / positive regulation of MAPK cascade / G protein-coupled receptor signaling pathway / axon / glutamatergic synapse / negative regulation of apoptotic process / extracellular space / extracellular region / cytoplasm Similarity search - Function | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | D'Ursi, A.M. / Scrima, M. / Esposito, C. / Campiglia, P. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2007 Title: Obestatin conformational features: a strategy to unveil obestatin's biological role? Authors: Scrima, M. / Campiglia, P. / Esposito, C. / Gomez-Monterrey, I. / Novellino, E. / D'Ursi, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jsi.cif.gz | 88.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jsi.ent.gz | 66.2 KB | Display | PDB format |
PDBx/mmJSON format | 2jsi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/js/2jsi ftp://data.pdbj.org/pub/pdb/validation_reports/js/2jsi | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1428.576 Da / Num. of mol.: 1 / Fragment: residues 86-98 / Source method: obtained synthetically / Details: The peptides were chemical synthesized / References: UniProt: Q9EQX0 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 2.7 mM [U-100% 2H] SDS, 27 mM [U-100% 2H] DPC, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 50 / Conformers submitted total number: 25 |