+Open data
-Basic information
Entry | Database: PDB / ID: 6dza | ||||||
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Title | Solution structure of Rbfox2 RRM mimetic peptide CPfox4 | ||||||
Components | CPfox4 | ||||||
Keywords | RNA BINDING PROTEIN / peptidomimetics / peptide structure / RNA recognition | ||||||
Biological species | synthetic construct (others) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Sun, Y.-T. / Shortridge, M.D. / Varani, G. | ||||||
Citation | Journal: Chembiochem / Year: 2019 Title: A Small Cyclic beta-Hairpin Peptide Mimics the Rbfox2 RNA Recognition Motif and Binds to the Precursor miRNA 20b. Authors: Sun, Y.T. / Shortridge, M.D. / Varani, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6dza.cif.gz | 54.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6dza.ent.gz | 38.2 KB | Display | PDB format |
PDBx/mmJSON format | 6dza.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dz/6dza ftp://data.pdbj.org/pub/pdb/validation_reports/dz/6dza | HTTPS FTP |
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-Related structure data
Related structure data | 6dz9C 6dzbC 6dzcC 6dzeC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1997.303 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 3.0 mg/mL CPfox4, 90% H2O/10% D2O / Label: sample_1 / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 3.0 mg/mL / Component: CPfox4 / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength: 10 mM potassium phosphate, 10 mM potassium chloride mM Label: condition_1 / pH: 6.5 / Pressure: 1 atm / Temperature: 278 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 4 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 |