- PDB-2jr2: Solution NMR structure of homodimer CPS_2611 from Colwellia psych... -
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Basic information
Entry
Database: PDB / ID: 2jr2
Title
Solution NMR structure of homodimer CPS_2611 from Colwellia psychrerythraea. Northeast Structural Genomics Consortium target CsR4.
Components
UPF0352 protein CPS_2611
Keywords
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / dimer / all alpha helix / homodimer / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG
Function / homology
Uncharacterised protein family UPF0352 / YejL-like superfamily / Protein of unknown function (DUF1414) / YejL-like / YejL-like / Orthogonal Bundle / Mainly Alpha / UPF0352 protein CPS_2611
Function and homology information
Biological species
Colwellia psychrerythraea (bacteria)
Method
SOLUTION NMR / simulated annealing, CNS water refinement
Mass: 8604.974 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Colwellia psychrerythraea (bacteria) / Strain: 34H / Gene: CPS_2611 / Plasmid: pET21 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pMGK / References: UniProt: Q481E4
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR / Details: homodimer, all helix
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D 1H-15N HSQC
1
2
1
2D 1H-13C HSQC
1
3
1
3D 1H-15N NOESY
1
4
1
3D 1H-13C NOESY aliph
1
5
1
3D HN(CA)CB
1
6
2
2D 1H-13C HSQC
1
7
3
4D 1H-13C NOESY
1
8
1
3DHBHA(CO)NH
1
9
1
3D HNHA
1
10
1
3DCBCA(CO)NH
1
11
1
3D HNCA
1
12
1
3DHN(CO)CA
1
13
1
3D HNCO
1
14
1
3DC(CO)NH
1
15
1
3D (H)CCH-COSY
1
16
1
3D (H)CCH-TOCSY
1
17
3
2D 1H-13C HSQC
1
18
3
2D 1H-15N HSQC
1
19
4
3D edited filtered 1H-13C NOESY
1
20
1
3D 1H-13C NOESY arom
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1 mM [U-13C; U-15N] protein, 100 mM sodium chloride, 20 mM ammonium acetate, 5 mM DTT, 0.02 % sodium azide, 5 mM calcium chloride, 95% H2O/5% D2O
95% H2O/5% D2O
2
1.1 mM [U-5% 13C; U-100% 15N] protein, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 5 mM DTT, 0.02 % sodium azide, 95% H2O/5% D2O
95% H2O/5% D2O
3
1 mM [U-13C; U-15N] protein, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 5 mM DTT, 0.02 % sodium azide, 100% D2O
100% D2O
4
0.5 mM [U-13C; U-15N] protein, 100 mM sodium chloride, 5 mM calcium chloride, 20 mM ammonium acetate, 5 mM DTT, 0.02 % sodium azide, 0.5 mM protein, 100% D2O