+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 2jp9 | ||||||
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タイトル | Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA | ||||||
要素 |
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キーワード | TRANSCRIPTION/DNA / DNA binding (デオキシリボ核酸) / nucleic acid recognition / residual dipolar coupling / zinc finger (ジンクフィンガー) / Metal-binding / Zinc-finger (ジンクフィンガー) / TRANSCRIPTION-DNA COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 Nephron development / posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / : / negative regulation of female gonad development ...Nephron development / posterior mesonephric tubule development / negative regulation of metanephric glomerular mesangial cell proliferation / positive regulation of metanephric ureteric bud development / thorax and anterior abdomen determination / metanephric epithelium development / regulation of animal organ formation / adrenal cortex formation / : / negative regulation of female gonad development / positive regulation of heart growth / visceral serous pericardium development / glomerular basement membrane development / diaphragm development / sex determination / mesenchymal to epithelial transition / metanephric mesenchyme development / metanephric S-shaped body morphogenesis / positive regulation of male gonad development / cellular response to gonadotropin stimulus / Transcriptional regulation of testis differentiation / gonad development / podocyte differentiation / cardiac muscle cell fate commitment / double-stranded methylated DNA binding / hemi-methylated DNA-binding / tissue development / male genitalia development / glomerulus development / camera-type eye development / C2H2 zinc finger domain binding / negative regulation of gene expression via chromosomal CpG island methylation / ureteric bud development / branching involved in ureteric bud morphogenesis / adrenal gland development / germ cell development / 脈管形成 / cellular response to cAMP / epithelial cell differentiation / RNA splicing / kidney development / negative regulation of cell growth / positive regulation of miRNA transcription / male gonad development / heart development / DNA-binding transcription activator activity, RNA polymerase II-specific / sequence-specific DNA binding / negative regulation of translation / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / nuclear speck / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / positive regulation of gene expression / 核小体 / regulation of transcription by RNA polymerase II / negative regulation of apoptotic process / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / RNA binding / zinc ion binding / 核質 / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) synthetic construct (人工物) | ||||||
手法 | 溶液NMR / molecular dynamics | ||||||
データ登録者 | Stoll, R. / Lee, B.M. / Debler, E.W. / Laity, J.H. / Wilson, I.A. / Dyson, H.J. / Wright, P.E. | ||||||
引用 | ジャーナル: J.Mol.Biol. / 年: 2007 タイトル: Structure of the wilms tumor suppressor protein zinc finger domain bound to DNA 著者: Stoll, R. / Lee, B.M. / Debler, E.W. / Laity, J.H. / Wilson, I.A. / Dyson, H.J. / Wright, P.E. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 2jp9.cif.gz | 1.2 MB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb2jp9.ent.gz | 1001.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 2jp9.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/jp/2jp9 ftp://data.pdbj.org/pub/pdb/validation_reports/jp/2jp9 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: DNA鎖 | 分子量: 5261.371 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物) |
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#2: DNA鎖 | 分子量: 5159.327 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) synthetic construct (人工物) |
#3: タンパク質 | 分子量: 14488.708 Da / 分子数: 1 / Fragment: residues 174-291 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: WT1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q4VXV4, UniProt: P19544*PLUS |
#4: 化合物 | ChemComp-ZN / |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 0.6 mM [U-100% 13C; U-100% 15N] wt1, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O |
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試料 | 濃度: 0.6 mM / 構成要素: wt1 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
試料状態 | イオン強度: 20 / pH: 6.7 / 圧: ambient / 温度: 310 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 800 MHz |
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-解析
NMR software |
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精密化 | 手法: molecular dynamics / ソフトェア番号: 1 | |||||||||
代表構造 | 選択基準: lowest energy | |||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 |