- PDB-2joc: Mouse Itch 3rd domain phosphorylated in T30 -
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IDまたはキーワード:
読み込み中...
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基本情報
登録情報
データベース: PDB / ID: 2joc
タイトル
Mouse Itch 3rd domain phosphorylated in T30
要素
Itchy E3 ubiquitin protein ligase
キーワード
LIGASE / Itch / WW / Phosphothreonine
機能・相同性
機能・相同性情報
regulation of protein deubiquitination / Downregulation of ERBB4 signaling / negative regulation of cytoplasmic pattern recognition receptor signaling pathway / Activated NOTCH1 Transmits Signal to the Nucleus / Regulation of necroptotic cell death / negative regulation of defense response to virus / Degradation of GLI1 by the proteasome / protein K29-linked ubiquitination / Hedgehog 'on' state / T cell anergy ...regulation of protein deubiquitination / Downregulation of ERBB4 signaling / negative regulation of cytoplasmic pattern recognition receptor signaling pathway / Activated NOTCH1 Transmits Signal to the Nucleus / Regulation of necroptotic cell death / negative regulation of defense response to virus / Degradation of GLI1 by the proteasome / protein K29-linked ubiquitination / Hedgehog 'on' state / T cell anergy / positive regulation of T cell anergy / RUNX1 regulates transcription of genes involved in differentiation of HSCs / CXCR chemokine receptor binding / CD4-positive, alpha-beta T cell proliferation / NOD1/2 Signaling Pathway / Antigen processing: Ubiquitination & Proteasome degradation / negative regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of JNK cascade / HECT-type E3 ubiquitin transferase / arrestin family protein binding / positive regulation of receptor catabolic process / negative regulation of NF-kappaB transcription factor activity / ubiquitin-like protein ligase binding / protein monoubiquitination / ligase activity / protein K63-linked ubiquitination / protein autoubiquitination / protein K48-linked ubiquitination / ribonucleoprotein complex binding / ubiquitin ligase complex / protein catabolic process / receptor internalization / protein polyubiquitination / positive regulation of protein catabolic process / ubiquitin-protein transferase activity / ubiquitin protein ligase activity / cell cortex / early endosome membrane / cytoplasmic vesicle / ubiquitin-dependent protein catabolic process / proteasome-mediated ubiquitin-dependent protein catabolic process / defense response to virus / protein ubiquitination / innate immune response / apoptotic process / negative regulation of apoptotic process / nucleoplasm / membrane / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能
E3 ubiquitin-protein ligase, SMURF1 type / HECT domain / HECT, E3 ligase catalytic domain / HECT-domain (ubiquitin-transferase) / HECT domain profile. / Domain Homologous to E6-AP Carboxyl Terminus with / C2 domain / Protein kinase C conserved region 2 (CalB) / WW domain / WW/rsp5/WWP domain signature. ...E3 ubiquitin-protein ligase, SMURF1 type / HECT domain / HECT, E3 ligase catalytic domain / HECT-domain (ubiquitin-transferase) / HECT domain profile. / Domain Homologous to E6-AP Carboxyl Terminus with / C2 domain / Protein kinase C conserved region 2 (CalB) / WW domain / WW/rsp5/WWP domain signature. / C2 domain / C2 domain profile. / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / C2 domain superfamily 類似検索 - ドメイン・相同性
分子量: 4356.688 Da / 分子数: 1 / 断片: WW 3 domain, sequence database residues 399-432 / 由来タイプ: 合成 詳細: The peptide was chemically synthesized. The sequence of the peptide is naturally found in Mus musculus (mouse). 参照: UniProt: Q8C863, 合成酵素; C-N結合を形成; 酸-D-アミノ酸リガーゼ(ペプチド合成)
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D 1H-1H TOCSY
2
2
1
2D 1H-1H TOCSY
1
3
1
2D 1H-1H NOESY
2
4
1
2D 1H-1H NOESY
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試料調製
詳細
内容: 1.0 mM PhosphoI3, 20 mM Sodium Phosphate, 100 mM NaCl, 0.02% v/v Sodium Azide, 90% H2O/10% D2O 溶媒系: 90% H2O/10% D2O
試料
濃度 (mg/ml)
構成要素
Solution-ID
1.0mM
PhosphoI3
1
20mM
SodiumPhosphate
1
100mM
NaCl
1
0.02v/v
SodiumAzide
1
試料状態
Conditions-ID
イオン強度
pH
圧 (kPa)
温度 (K)
1
0.4
6.5
ambient
280K
2
0.4
6.5
ambient
285K
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz
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解析
NMR software
名称
開発者
分類
TopSpin
BrukerBiospin
collection
NMRPipe
Delaglio, Grzesiek, Vuister, Zhu, PfeiferandBax
解析
ARIA
Linge, O'DonoghueandNilges
構造決定
ARIA
Linge, O'DonoghueandNilges
精密化
精密化
手法: molecular dynamics / ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: all calculated structures submitted 計算したコンフォーマーの数: 7 / 登録したコンフォーマーの数: 7