+
Open data
-
Basic information
Entry | Database: PDB / ID: 2jg3 | ||||||
---|---|---|---|---|---|---|---|
Title | MtaqI with BAZ | ||||||
![]() |
| ||||||
![]() | TRANSFERASE/DNA / TRANSFERASE-DNA COMPLEX / DNA / TRANSFERASE / BASE FLIPPING / RESTRICTION SYSTEM | ||||||
Function / homology | ![]() site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / DNA restriction-modification system / methylation / DNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pljevaljcic, G. / Scheidig, A.J. / Weinhold, E. | ||||||
![]() | ![]() Title: Quantitative Labeling of Long Plasmid DNA with Nanometer Precision. Authors: Pljevaljcic, G. / Schmidt, F. / Scheidig, A.J. / Lurz, R. / Weinhold, E. #1: ![]() Title: Structure of the N6-Adenine DNA Methyltransferase M.TaqI in Complex with DNA and a Cofactor Analog Authors: Goedecke, K. / Pignot, M. / Goody, R.S. / Scheidig, A.J. / Weinhold, E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 212.4 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 163.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 40.1 KB | Display | |
Data in CIF | ![]() | 58.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1g38S S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
-Protein , 1 types, 2 molecules AD
#1: Protein | Mass: 47931.195 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: ADENINE-SPECIFIC METHYLTRANSFERASE TAQI, M. TAQI / Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P14385, site-specific DNA-methyltransferase (adenine-specific) |
---|
-DNA chain , 2 types, 4 molecules BECF
#2: DNA chain | Mass: 3051.001 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: DNA chain | Mass: 3052.046 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
---|
-Non-polymers , 4 types, 686 molecules ![](data/chem/img/BA2.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.51 % / Description: NONE |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.3 Details: 3 MICROLITERS CRYSTALLIZATION BUFFER (10 MM TRIS/HCL, 300 MM NACL, PH 7.3) CONTAINING THE COMPLEX PLUS 1 MICROLITER RESERVOIR SOLUTION (100 MM KCL, 100 MM MGCL2, 6% ISOPROPANOL, 50 MM SODIUM ...Details: 3 MICROLITERS CRYSTALLIZATION BUFFER (10 MM TRIS/HCL, 300 MM NACL, PH 7.3) CONTAINING THE COMPLEX PLUS 1 MICROLITER RESERVOIR SOLUTION (100 MM KCL, 100 MM MGCL2, 6% ISOPROPANOL, 50 MM SODIUM CACODYLATE, PH 6.0), VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 21, 2001 / Details: ID14-1 (MIRROR) |
Radiation | Monochromator: ID14-1 (MIRROR) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→19.79 Å / Num. obs: 72769 / % possible obs: 99.4 % / Observed criterion σ(I): 0 / Redundancy: 4.522 % / Rmerge(I) obs: 0.09 / Net I/σ(I): 14.62 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 2.88 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 4.94 / % possible all: 97.7 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1G38 Resolution: 1.9→19.73 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.924 / SU B: 6.695 / SU ML: 0.104 / TLS residual ADP flag: UNVERIFIED / Cross valid method: THROUGHOUT / ESU R: 0.165 / ESU R Free: 0.148 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGIONS (LINKER AND BIOTIN) WERE MODELED STEREOCHEMICALLY
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 12.19 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→19.73 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
|