- PDB-2j9i: Lengsin is a survivor of an ancient family of class I glutamine s... -
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基本情報
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データベース: PDB / ID: 2j9i
タイトル
Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.
要素
GLUTAMATE-AMMONIA LIGASE DOMAIN-CONTAINING PROTEIN 1
ジャーナル: Structure / 年: 2006 タイトル: Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens. 著者: Keith Wyatt / Helen E White / Luchun Wang / Orval A Bateman / Christine Slingsby / Elena V Orlova / Graeme Wistow / 要旨: Lengsin is a major protein of the vertebrate eye lens. It belongs to the hitherto purely prokaryotic GS I branch of the glutamine synthetase (GS) superfamily, but has no enzyme activity. Like the ...Lengsin is a major protein of the vertebrate eye lens. It belongs to the hitherto purely prokaryotic GS I branch of the glutamine synthetase (GS) superfamily, but has no enzyme activity. Like the taxon-specific crystallins, Lengsin is the result of the recruitment of an ancient enzyme to a noncatalytic role in the vertebrate lens. Cryo-EM and modeling studies of Lengsin show a dodecamer structure with important similarities and differences with prokaryotic GS I structures. GS homology regions of Lengsin are well conserved, but the N-terminal domain shows evidence of dynamic evolutionary changes. Compared with birds and fish, most mammals have an additional exon corresponding to part of the N-terminal domain; however, in human, this is a nonfunctional pseudoexon. Genes related to Lengsin are also present in the sea urchin, suggesting that this branch of the GS I family, supplanted by GS II enzymes in vertebrates, has an ancient role in metazoans.
履歴
登録
2006年11月9日
登録サイト: PDBE / 処理サイト: PDBE
改定 1.0
2006年12月13日
Provider: repository / タイプ: Initial release
改定 1.1
2013年8月7日
Group: Derived calculations / Other ...Derived calculations / Other / Refinement description / Source and taxonomy / Version format compliance
SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
モード: BRIGHT FIELD / 倍率(公称値): 50000 X / 最大 デフォーカス(公称値): 320 nm / 最小 デフォーカス(公称値): 170 nm
撮影
フィルム・検出器のモデル: KODAK SO-163 FILM
放射波長
相対比: 1
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解析
EMソフトウェア
ID
名称
カテゴリ
1
URO
モデルフィッティング
2
IMAGIC
3次元再構成
CTF補正
詳細: PHASE FLIPPING
対称性
点対称性: I (正20面体型対称)
3次元再構成
手法: EXACT-FILTER BACK PROJECTION / 解像度: 17 Å / ピクセルサイズ(公称値): 1.4 Å / ピクセルサイズ(実測値): 1.45 Å 詳細: THE HOMOLOGY MODEL WAS MINIMIZED IN AMBER 8 PRIOR TO MANUAL FITTING 対称性のタイプ: POINT