GPER1 signaling / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / Factors involved in megakaryocyte development and platelet production / Hedgehog 'off' state / PKA activation / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events ...GPER1 signaling / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / Factors involved in megakaryocyte development and platelet production / Hedgehog 'off' state / PKA activation / PKA activation in glucagon signalling / CREB1 phosphorylation through the activation of Adenylate Cyclase / DARPP-32 events / PKA activation / Vasopressin regulates renal water homeostasis via Aquaporins / GPER1 signaling / Hedgehog 'off' state / Factors involved in megakaryocyte development and platelet production / cAMP-dependent protein kinase regulator activity / nucleotide-activated protein kinase complex / Vasopressin regulates renal water homeostasis via Aquaporins / cAMP-dependent protein kinase inhibitor activity / beta-2 adrenergic receptor binding / cAMP-dependent protein kinase complex / small molecule binding / plasma membrane raft / protein kinase A catalytic subunit binding / cAMP binding / T-tubule / regulation of protein phosphorylation / modulation of chemical synaptic transmission / protein domain specific binding / centrosome / ubiquitin protein ligase binding / glutamatergic synapse / synapse / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / identical protein binding / plasma membrane / cytoplasm / cytosol Similarity search - Function
cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / cAMP-dependent Protein Kinase, Chain A / : / cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / Cyclic nucleotide-binding domain signature 2. / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site ...cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / cAMP-dependent Protein Kinase, Chain A / : / cAMP-dependent protein kinase regulatory subunit / cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain / Regulatory subunit of type II PKA R-subunit / RIIalpha, Regulatory subunit portion of type II PKA R-subunit / Cyclic nucleotide-binding domain signature 2. / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site / Cyclic nucleotide-monophosphate binding domain / Cyclic nucleotide-binding domain / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Cyclic nucleotide-binding domain superfamily / RmlC-like jelly roll fold / Up-down Bundle / Mainly Alpha Similarity search - Domain/homology
cAMP-dependent protein kinase type II-alpha regulatory subunit / cAMP-dependent protein kinase type II-alpha regulatory subunit Similarity search - Component
Biological species
MUS MUSCULUS (house mouse)
Method
X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2.2 Å
A: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II B: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II C: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II D: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II E: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II F: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II G: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II H: CAMP-DEPENDENT PROTEIN KINASE REGULATORY SUBUNIT II
Resolution: 2.2→30 Å / Num. obs: 23415 / % possible obs: 100 % / Observed criterion σ(I): 2.5 / Redundancy: 28 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 11.6
Reflection shell
Resolution: 2.2→2.28 Å / Rmerge(I) obs: 0.24 / % possible all: 88.2
-
Processing
Software
Name
Version
Classification
REFMAC
5.2.0019
refinement
MOSFLM
datareduction
SCALEPACK
datascaling
SHELXS
phasing
Refinement
Method to determine structure: MAD / Resolution: 2.2→29.58 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.879 / SU B: 5.517 / SU ML: 0.145 / Cross valid method: THROUGHOUT / ESU R: 0.306 / ESU R Free: 0.241 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES VISIBLE C-TERMINAL TO POSITION 46 ARE PART OF AN UNCLEAVED 6HIS TAG
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.272
1970
8.4 %
RANDOM
Rwork
0.215
-
-
-
obs
0.22
21445
100 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK