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Yorodumi- PDB-2iyg: Dark state structure of the BLUF domain of the rhodobacterial pro... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2iyg | ||||||
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Title | Dark state structure of the BLUF domain of the rhodobacterial protein AppA | ||||||
Components | APPA, ANTIREPRESSOR OF PPSR, SENSOR OF BLUE LIGHT | ||||||
Keywords | SIGNAL TRANSDUCTION | ||||||
Function / homology | Function and homology information | ||||||
Biological species | RHODOBACTER SPHAEROIDES (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.3 Å | ||||||
Authors | Jung, A. / Reinstein, J. / Domratcheva, T. / Shoeman, R.-L. / Schlichting, I. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Crystal Structures of the Appa Bluf Domain Photoreceptor Provide Insights Into Blue Light- Mediated Signal Transduction. Authors: Jung, A. / Reinstein, J. / Domratcheva, T. / Shoeman, R.-L. / Schlichting, I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2iyg.cif.gz | 54.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2iyg.ent.gz | 43.8 KB | Display | PDB format |
PDBx/mmJSON format | 2iyg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/iy/2iyg ftp://data.pdbj.org/pub/pdb/validation_reports/iy/2iyg | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 13919.674 Da / Num. of mol.: 2 / Fragment: BLUF DOMAIN, RESIDUES 1-124 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) RHODOBACTER SPHAEROIDES (bacteria) / Plasmid: PET28 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q3J677 #2: Chemical | #3: Chemical | ChemComp-DTU / ( | #4: Chemical | ChemComp-DTT / | #5: Water | ChemComp-HOH / | Compound details | ENGINEERED | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.5 % |
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Crystal grow | pH: 8.5 / Details: pH 8.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.92524 |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92524 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→10 Å / Num. obs: 17447 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 6 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 15.4 |
Reflection shell | Resolution: 2.3→2.36 Å / Redundancy: 6 % / Rmerge(I) obs: 0.26 / Mean I/σ(I) obs: 5.5 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.3→9.99 Å / Cor.coef. Fo:Fc: 0.942 / Cor.coef. Fo:Fc free: 0.934 / SU B: 12.156 / SU ML: 0.15 / TLS residual ADP flag: UNVERIFIED / Cross valid method: THROUGHOUT / ESU R: 0.268 / ESU R Free: 0.202 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Displacement parameters | Biso mean: 22.96 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→9.99 Å
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Refine LS restraints |
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