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- PDB-2ixp: Crystal structure of the Pp2A phosphatase activator Ypa1 PTPA1 in... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2ixp | |||||||||
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Title | Crystal structure of the Pp2A phosphatase activator Ypa1 PTPA1 in complex with model substrate | |||||||||
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![]() | ISOMERASE/ISOMERASE INHIBITOR / PP2A PHOSPHATASE ACTIVATOR PROLYL ISOMERASE PTPA / PROLYL CONTAINING PEPTIDE SUBSTRATE ANALOG / NUCLEAR PROTEIN / ISOMERASE-ISOMERASE INHIBITOR COMPLEX | |||||||||
Function / homology | ![]() protein tyrosine phosphatase activator activity / protein phosphatase type 2A complex / protein phosphatase regulator activity / TOR signaling / mitotic spindle organization / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / autophagy / G1/S transition of mitotic cell cycle / DNA repair ...protein tyrosine phosphatase activator activity / protein phosphatase type 2A complex / protein phosphatase regulator activity / TOR signaling / mitotic spindle organization / peptidylprolyl isomerase / peptidyl-prolyl cis-trans isomerase activity / autophagy / G1/S transition of mitotic cell cycle / DNA repair / chromatin / regulation of transcription by RNA polymerase II / nucleus / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() SYNTHETIC CONSTRUCT (others) | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Leulliot, N. / Vicentini, G. / Jordens, J. / Quevillon-Cheruel, S. / Schiltz, M. / Barford, D. / Van Tilbeurgh, H. / Goris, J. | |||||||||
![]() | ![]() Title: Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity. Authors: Leulliot, N. / Vicentini, G. / Jordens, J. / Quevillon-Cheruel, S. / Schiltz, M. / Barford, D. / van Tilbeurgh, H. / Goris, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 234.8 KB | Display | ![]() |
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PDB format | ![]() | 185.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 480 KB | Display | ![]() |
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Full document | ![]() | 543.2 KB | Display | |
Data in XML | ![]() | 46.8 KB | Display | |
Data in CIF | ![]() | 65.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ixmC ![]() 2ixnC ![]() 2ixoSC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 37463.945 Da / Num. of mol.: 4 / Fragment: 1-317 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Protein/peptide | #3: Chemical | ChemComp-SO4 / #4: Chemical | Compound details | THE PEPTIDE IS A PROLINE CONTAINING | Sequence details | RESIDUES 1-317 AND C-TERMINAL HIS TAG | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.07 Å3/Da / Density % sol: 59.68 % |
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Crystal grow | pH: 6.5 Details: 1.8MM PEPTIDE, 1M AMMONIUM SULFATE, 3% GLYCEROL, 0.1M ADA PH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. obs: 42938 / % possible obs: 99.9 % / Observed criterion σ(I): 2 / Redundancy: 3.8 % / Rmerge(I) obs: 0.2 / Net I/σ(I): 5.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2IXO Resolution: 2.8→29.12 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.874 / SU B: 40.408 / SU ML: 0.362 / TLS residual ADP flag: UNVERIFIED / Cross valid method: THROUGHOUT / ESU R Free: 0.435 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 2.49 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→29.12 Å
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Refine LS restraints |
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