- PDB-2ih5: Crystal structure of the adenine-specific DNA methyltransferase M... -
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基本情報
登録情報
データベース: PDB / ID: 2ih5
タイトル
Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target base partner position
要素
5'-D(*GP*AP*CP*AP*(3DR)P*CP*GP*(6MA)P*AP*C)-3'
5'-D(*GP*TP*TP*CP*GP*AP*TP*GP*TP*C)-3'
Modification methylase TaqI
キーワード
TRANSFERASE/DNA / DNA / DNA methyltransferase / target base partner / abasic site analog / base flipping / nucleotide flipping / TRANSFERASE-DNA COMPLEX
機能・相同性
機能・相同性情報
site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / DNA restriction-modification system / methylation / DNA binding 類似検索 - 分子機能
Adenine-n6-DNA-methyltransferase Taqi, Chain A, domain 2 / Type II restriction enzyme and methyltransferase RM.Eco57I-like / N6 adenine-specific DNA methyltransferase, TaqI class / N6 adenine-specific DNA methyltransferase, TaqI class, C-terminal / Eco57I restriction-modification methylase / Adenine-n6-DNA-methyltransferase TaqI; Chain A, domain 2 / TaqI-like C-terminal specificity domain / TaqI-like C-terminal specificity domain / N-6 Adenine-specific DNA methylases signature. / DNA methylase, N-6 adenine-specific, conserved site ...Adenine-n6-DNA-methyltransferase Taqi, Chain A, domain 2 / Type II restriction enzyme and methyltransferase RM.Eco57I-like / N6 adenine-specific DNA methyltransferase, TaqI class / N6 adenine-specific DNA methyltransferase, TaqI class, C-terminal / Eco57I restriction-modification methylase / Adenine-n6-DNA-methyltransferase TaqI; Chain A, domain 2 / TaqI-like C-terminal specificity domain / TaqI-like C-terminal specificity domain / N-6 Adenine-specific DNA methylases signature. / DNA methylase, N-6 adenine-specific, conserved site / Vaccinia Virus protein VP39 / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Alpha-Beta Complex / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
5'-DEOXY-5'-[2-(AMINO)ETHYLTHIO]ADENOSINE / DNA / Type II methyltransferase M.TaqI 類似検索 - 構成要素
解像度: 1.8→19.82 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.942 / SU B: 2.516 / SU ML: 0.079 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.121 / ESU R Free: 0.117 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.20196
2203
5 %
RANDOM
Rwork
0.16123
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obs
0.16329
44043
97.76 %
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all
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45053
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK