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- PDB-2iez: Crystal Structure of mouse Rab27b bound to GDP in monoclinic spac... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2iez | ||||||
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Title | Crystal Structure of mouse Rab27b bound to GDP in monoclinic space group | ||||||
![]() | Ras-related protein Rab-27B | ||||||
![]() | SIGNALING PROTEIN / Rab27 / GTPase / Rab / GDP / swapping | ||||||
Function / homology | ![]() RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / neurotransmitter receptor transport to postsynaptic membrane / Golgi vesicle fusion to target membrane / zymogen granule membrane / anterograde axonal protein transport / Platelet degranulation / exocytic vesicle / multivesicular body sorting pathway / myosin V binding ...RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / neurotransmitter receptor transport to postsynaptic membrane / Golgi vesicle fusion to target membrane / zymogen granule membrane / anterograde axonal protein transport / Platelet degranulation / exocytic vesicle / multivesicular body sorting pathway / myosin V binding / regulation of exocytosis / multivesicular body membrane / Golgi stack / trans-Golgi network transport vesicle / endocytic recycling / vesicle docking involved in exocytosis / exocytosis / positive regulation of exocytosis / synaptic vesicle endocytosis / protein secretion / cilium assembly / axon cytoplasm / small monomeric GTPase / G protein activity / secretory granule / protein localization to plasma membrane / synaptic vesicle membrane / GDP binding / melanosome / late endosome / dendritic spine / apical plasma membrane / protein domain specific binding / GTPase activity / GTP binding / Golgi apparatus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chavas, L.M.G. / Torii, S. / Kamikubo, H. / Kawasaki, M. / Ihara, K. / Kato, R. / Kataoka, M. / Izumi, T. / Wakatsuki, S. | ||||||
![]() | ![]() Title: Structure of the small GTPase Rab27b shows an unexpected swapped dimer Authors: Chavas, L.M.G. / Torii, S. / Kamikubo, H. / Kawasaki, M. / Ihara, K. / Kato, R. / Kataoka, M. / Izumi, T. / Wakatsuki, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.6 KB | Display | ![]() |
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PDB format | ![]() | 122.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 28.1 KB | Display | |
Data in CIF | ![]() | 37.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2ieySC ![]() 2if0C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological unit is a monomer. There are 4 biological units in the asymmetric unit (chain A and chain B and chain H and chain I) |
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Components
#1: Protein | Mass: 24717.943 Da / Num. of mol.: 4 / Fragment: soluble domain / Mutation: Q78L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-GDP / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.69 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion / pH: 7 Details: MPD, CaCl2, Hepes, pH 7.0, VAPOR DIFFUSION, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 2, 2005 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.71→84.21 Å / Num. obs: 20069 / % possible obs: 86.2 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Redundancy: 3 % / Rmerge(I) obs: 0.121 / Χ2: 1.019 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.484 / Num. unique all: 1770 / % possible all: 76.4 |
-Phasing
Phasing MR |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2IEY Resolution: 2.8→35.6 Å / Cor.coef. Fo:Fc: 0.898 / Cor.coef. Fo:Fc free: 0.815 / SU B: 23.271 / SU ML: 0.464 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.618 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.422 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→35.6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.872 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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