+Open data
-Basic information
Entry | Database: PDB / ID: 2ibb | ||||||
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Title | Crystal Structure of the First and Second FNIII Domains of Ihog | ||||||
Components | CG9211-PA | ||||||
Keywords | PROTEIN BINDING / ihog / hedgehog / fibronectin type III | ||||||
Function / homology | Function and homology information maintenance of protein location in extracellular region / Myogenesis / cuticle pattern formation / Ligand-receptor interactions / cytoneme / Assembly of the 'signalling complexes' / Signaling by ROBO receptors / : / wing disc pattern formation / wing disc anterior/posterior pattern formation ...maintenance of protein location in extracellular region / Myogenesis / cuticle pattern formation / Ligand-receptor interactions / cytoneme / Assembly of the 'signalling complexes' / Signaling by ROBO receptors / : / wing disc pattern formation / wing disc anterior/posterior pattern formation / compound eye development / hedgehog family protein binding / homotypic cell-cell adhesion / segment specification / patched binding / smoothened signaling pathway / coreceptor activity / cell-cell adhesion / heparin binding / cell surface / protein homodimerization activity / membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | McLellan, J.S. / Leahy, D.J. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2006 Title: Structure of a heparin-dependent complex of Hedgehog and Ihog. Authors: McLellan, J.S. / Yao, S. / Zheng, X. / Geisbrecht, B.V. / Ghirlando, R. / Beachy, P.A. / Leahy, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ibb.cif.gz | 54.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ibb.ent.gz | 39.1 KB | Display | PDB format |
PDBx/mmJSON format | 2ibb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ib/2ibb ftp://data.pdbj.org/pub/pdb/validation_reports/ib/2ibb | HTTPS FTP |
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-Related structure data
Related structure data | 2ibgC 2ic2C 1fnfS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 24161.174 Da / Num. of mol.: 1 / Fragment: Extracellular FNIII Domains Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: iHog, CG9211 / Plasmid: pT7HMT / Production host: Escherichia coli (E. coli) / Strain (production host): B834(DE3) / References: UniProt: Q9VM64 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.02 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.9 Details: 0.05M Tris-HCl pH 7.9, 23% PEG 3350, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-BM-C / Wavelength: 0.9 Å | ||||||
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 29, 2004 | ||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 | ||||||
Reflection | Resolution: 2.4→50 Å / Num. obs: 8851 / % possible obs: 98.1 % / Redundancy: 5 % / Rmerge(I) obs: 0.126 / Χ2: 1.412 / Net I/σ(I): 7.5 | ||||||
Reflection shell | Resolution: 2.4→2.49 Å / Redundancy: 5.2 % / Rmerge(I) obs: 0.801 / Num. unique all: 879 / Χ2: 0.99 / % possible all: 99.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: First FNIII domain Ihog and PDB Entry: 1FNF domain 7 Resolution: 2.4→21.57 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Bsol: 23.396 Å2 | ||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.263 Å2
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Refinement step | Cycle: LAST / Resolution: 2.4→21.57 Å
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Refine LS restraints |
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Xplor file |
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