溶液NMR / structures were subjected to 60 ps (15 fs time steps) of restrained molecular dynamics in torsion angle space, 90 ps of slow cooling. Finally 30 ps (5 fs time steps) of restrained molecular dynamics in Cartesian coordinate space were performed
内容: 1 mM GAAA tetraloop receptor RNA, 10 mM MgCl2, 2 mM cobalt hexaamine, 50 mM NaCl, pH 7.0; 90% H2O 10 %D2O 溶媒系: 90% H2O/10% D2O
試料状態
pH: 7 / 圧: ambient / 温度: 283 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker DMX
Bruker
DMX
750
1
Varian INOVA
Varian
INOVA
900
2
Bruker DMX
Bruker
DMX
600
3
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解析
NMR software
名称
バージョン
開発者
分類
CNS
1
BrungerA. T. etall
精密化
X-PLOR
2.11
精密化
XwinNMR
2.6
解析
Sparky
3.111
データ解析
精密化
手法: structures were subjected to 60 ps (15 fs time steps) of restrained molecular dynamics in torsion angle space, 90 ps of slow cooling. Finally 30 ps (5 fs time steps) of restrained molecular ...手法: structures were subjected to 60 ps (15 fs time steps) of restrained molecular dynamics in torsion angle space, 90 ps of slow cooling. Finally 30 ps (5 fs time steps) of restrained molecular dynamics in Cartesian coordinate space were performed ソフトェア番号: 1
代表構造
選択基準: lowest energy
NMRアンサンブル
コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20