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Yorodumi- PDB-2i0q: Crystal structure of a telomere single-strand DNA-protein complex... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2i0q | ||||||
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Title | Crystal structure of a telomere single-strand DNA-protein complex from O. nova with full-length alpha and beta telomere proteins | ||||||
Components |
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Keywords | structural protein/DNA / SINGLE STRAND DNA-PROTEIN COMPLEX / structural protein-DNA COMPLEX | ||||||
Function / homology | Function and homology information telomere cap complex / telomerase inhibitor activity / regulation of telomere maintenance via telomerase / nuclear telomere cap complex / single-stranded telomeric DNA binding / G-rich strand telomeric DNA binding / telomere capping / protein-containing complex / nucleus Similarity search - Function | ||||||
Biological species | Sterkiella nova (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.91 Å | ||||||
Authors | Horvath, M.P. / Buczek, P. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2006 Title: Structural reorganization and the cooperative binding of single-stranded telomere DNA in Sterkiella nova. Authors: Buczek, P. / Horvath, M.P. #1: Journal: Cell(Cambridge,Mass.) / Year: 1998 Title: Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA Authors: Horvath, M.P. / Schweiker, V.L. / Bevilacqua, J.M. / Ruggles, J.A. / Schultz, S.C. #2: Journal: J.Mol.Biol. / Year: 2001 Title: DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex Authors: Horvath, M.P. / Schultz, S.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2i0q.cif.gz | 167 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2i0q.ent.gz | 126.4 KB | Display | PDB format |
PDBx/mmJSON format | 2i0q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2i0q_validation.pdf.gz | 461.2 KB | Display | wwPDB validaton report |
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Full document | 2i0q_full_validation.pdf.gz | 472.3 KB | Display | |
Data in XML | 2i0q_validation.xml.gz | 29.4 KB | Display | |
Data in CIF | 2i0q_validation.cif.gz | 42.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i0/2i0q ftp://data.pdbj.org/pub/pdb/validation_reports/i0/2i0q | HTTPS FTP |
-Related structure data
Related structure data | 1jb7S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-DNA chain , 1 types, 1 molecules D
#1: DNA chain | Mass: 3476.254 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: 3'-terminal single strand DNA fragment from Oxytricha nova (Sterkiella nova) macronuclear telomeres |
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-Telomere-binding protein ... , 2 types, 2 molecules AB
#2: Protein | Mass: 56168.301 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sterkiella nova (eukaryote) / Gene: MAC-56A / Plasmid: pKKT7e / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) pLysS / References: UniProt: P29549 |
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#3: Protein | Mass: 41527.527 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sterkiella nova (eukaryote) / Gene: MAC-41A / Plasmid: pKKT7e / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) pLysS / References: UniProt: P16458 |
-Non-polymers , 3 types, 315 molecules
#4: Chemical | ChemComp-CL / #5: Chemical | ChemComp-EDO / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.63 Å3/Da / Density % sol: 53.23 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 5.75 Details: 21% (w/v) PEG-4000, 10% (v/v) ethylene glycol, 1.5 M sodium chloride, 40 mM MES, 2 mM DTT, 0.02% sodium azide, VAPOR DIFFUSION, HANGING DROP, pH 5.75, temperature 277.15K | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 12.3.1 / Wavelength: 1.11587 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 1, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.11587 Å / Relative weight: 1 |
Reflection | Resolution: 1.91→50 Å / Num. all: 86086 / Num. obs: 85120 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 8.1 % / Biso Wilson estimate: 47.6 Å2 / Rsym value: 0.041 / Net I/σ(I): 38.8 |
Reflection shell | Resolution: 1.91→1.98 Å / Redundancy: 5.3 % / Mean I/σ(I) obs: 3.5 / Rsym value: 0.46 / % possible all: 95.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1JB7 Resolution: 1.91→50 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): -3 / Stereochemistry target values: Engh & Huber Details: Maximum likelihood refinement with intensities as target function (mli)
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Solvent computation | Solvent model: CNS SOLVE / Bsol: 30.2205 Å2 / ksol: 0.320563 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.3 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.91→50 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION
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