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- PDB-2hmw: Square-shaped octameric ring structure of an RCK domain with ATP bound -
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Open data
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Basic information
Entry | Database: PDB / ID: 2hmw | ||||||
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Title | Square-shaped octameric ring structure of an RCK domain with ATP bound | ||||||
![]() | YuaA protein | ||||||
![]() | TRANSPORT PROTEIN / RCK / KTN / KTR / KTRA / KTRAB / membrane protein / ion transporter / symporter | ||||||
Function / homology | ![]() monoatomic cation transmembrane transporter activity / potassium ion transport / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Albright, R.A. / Morais-Cabral, J.H. | ||||||
![]() | ![]() Title: The RCK Domain of the KtrAB K(+) Transporter: Multiple Conformations of an Octameric Ring. Authors: Albright, R.A. / Ibar, J.L. / Kim, C.U. / Gruner, S.M. / Morais-Cabral, J.H. #1: ![]() Title: A Mechanism of Regulating Transmembrane Potassium Flux through a Ligand-Mediated Conformational Switch Authors: Roosild, T.P. / Miller, S. / Booth, I.R. / Choe, S. #2: ![]() Title: Crystal structure and mechanism of a calcium-gated potassium channel Authors: Jiang, Y. / Lee, A. / Chen, J. / Cadene, M. / Chait, B.T. / MacKinnon, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.4 KB | Display | ![]() |
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PDB format | ![]() | 50.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 981.1 KB | Display | ![]() |
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Full document | ![]() | 994.3 KB | Display | |
Data in XML | ![]() | 14.5 KB | Display | |
Data in CIF | ![]() | 18.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2hmsC ![]() 2hmtC ![]() 2hmuC ![]() 2hmvC ![]() 1lsuS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The biological assembly is an octameric ring generated from the asymmetric unit dimer using these symmetry operators: X, Y, Z and Y, 1-X, Z and 1-X, 1-Y, Z and 1-Y, X, Z |
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Components
#1: Protein | Mass: 16076.447 Da / Num. of mol.: 2 / Fragment: RCK CORE DOMAIN (KTN), residues 1-144 / Mutation: C22V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.06 Å3/Da / Density % sol: 59.76 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.8 Details: 500mM MgCl2, 50mM Tris pH 8.8, 8.5% (w/v) PEG 2000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 15, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 3→50 Å / Num. all: 8258 / Num. obs: 8231 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9.9 % / Rmerge(I) obs: 0.1 / Χ2: 1.007 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 3→3.11 Å / Redundancy: 10.2 % / Rmerge(I) obs: 0.731 / Num. unique all: 799 / Χ2: 1.007 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: single domain from pdb entry 1LSU Resolution: 3→50 Å / FOM work R set: 0.756 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 50.849 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 84.146 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→50 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 17
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Xplor file |
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