+Open data
-Basic information
Entry | Database: PDB / ID: 2hm8 | ||||||
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Title | Solution Structure of the C-terminal MA-3 domain of Pdcd4 | ||||||
Components | Pdcd4 C-terminal MA-3 domain | ||||||
Keywords | APOPTOSIS / atypical HEAT domain | ||||||
Function / homology | Function and homology information epithelial to mesenchymal transition involved in cardiac fibroblast development / negative regulation of myofibroblast differentiation / negative regulation of vascular associated smooth muscle cell differentiation / regulation of protein metabolic process / positive regulation of smooth muscle cell apoptotic process / negative regulation of JUN kinase activity / positive regulation of endothelial cell apoptotic process / response to alkaloid / positive regulation of vascular associated smooth muscle cell apoptotic process / negative regulation of vascular associated smooth muscle cell proliferation ...epithelial to mesenchymal transition involved in cardiac fibroblast development / negative regulation of myofibroblast differentiation / negative regulation of vascular associated smooth muscle cell differentiation / regulation of protein metabolic process / positive regulation of smooth muscle cell apoptotic process / negative regulation of JUN kinase activity / positive regulation of endothelial cell apoptotic process / response to alkaloid / positive regulation of vascular associated smooth muscle cell apoptotic process / negative regulation of vascular associated smooth muscle cell proliferation / negative regulation of cytokine production involved in inflammatory response / BMP signaling pathway / response to hormone / positive regulation of inflammatory response / positive regulation of non-canonical NF-kappaB signal transduction / cellular response to lipopolysaccharide / negative regulation of DNA-templated transcription / apoptotic process / negative regulation of apoptotic process / RNA binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Waters, L.C. / Veverka, V. / Bohm, M. / Muskett, F.W. / Choong, P.T. / Klempnauer, K.H. / Carr, M.D. | ||||||
Citation | Journal: Oncogene / Year: 2007 Title: Structure of the C-terminal MA-3 domain of the tumour suppressor protein Pdcd4 and characterization of its interaction with eIF4A Authors: Waters, L.C. / Veverka, V. / Bohm, M. / Schmedt, T. / Choong, P.T. / Muskett, F.W. / Klempnauer, K.H. / Carr, M.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hm8.cif.gz | 2.3 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2hm8.ent.gz | 2 MB | Display | PDB format |
PDBx/mmJSON format | 2hm8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hm/2hm8 ftp://data.pdbj.org/pub/pdb/validation_reports/hm/2hm8 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15504.788 Da / Num. of mol.: 1 / Fragment: MA-3 protein Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Pdcd4, MA-3 / Plasmid: pGEX6P2 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q61823 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 150mM / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 49 |