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- PDB-1b8q: SOLUTION STRUCTURE OF THE EXTENDED NEURONAL NITRIC OXIDE SYNTHASE... -

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Basic information

Entry
Database: PDB / ID: 1b8q
TitleSOLUTION STRUCTURE OF THE EXTENDED NEURONAL NITRIC OXIDE SYNTHASE PDZ DOMAIN COMPLEXED WITH AN ASSOCIATED PEPTIDE
Components
  • PROTEIN (HEPTAPEPTIDE)
  • PROTEIN (NEURONAL NITRIC OXIDE SYNTHASE)
KeywordsOXIDOREDUCTASE / PDZ DOMAIN / NNOS / NITRIC OXIDE SYNTHASE
Function / homology
Function and homology information


Nitric oxide stimulates guanylate cyclase / synaptic signaling by nitric oxide / negative regulation of hepatic stellate cell contraction / positive regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / ROS and RNS production in phagocytes / negative regulation of iron ion transmembrane transport / negative regulation of vasoconstriction / Ion homeostasis / azurophil granule / retrograde trans-synaptic signaling by nitric oxide ...Nitric oxide stimulates guanylate cyclase / synaptic signaling by nitric oxide / negative regulation of hepatic stellate cell contraction / positive regulation of adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / ROS and RNS production in phagocytes / negative regulation of iron ion transmembrane transport / negative regulation of vasoconstriction / Ion homeostasis / azurophil granule / retrograde trans-synaptic signaling by nitric oxide / positive regulation of sodium ion transmembrane transport / negative regulation of cytosolic calcium ion concentration / response to nitric oxide / postsynaptic specialization, intracellular component / postsynaptic density, intracellular component / positive regulation of the force of heart contraction / cadmium ion binding / negative regulation of serotonin uptake / behavioral response to cocaine / striated muscle contraction / peptidyl-cysteine S-nitrosylation / negative regulation of potassium ion transport / calyx of Held / negative regulation of calcium ion transport / regulation of sodium ion transport / multicellular organismal response to stress / negative regulation of insulin secretion / regulation of neurogenesis / sodium channel regulator activity / negative regulation of heart contraction / negative regulation of hydrolase activity / response to vitamin E / regulation of heart contraction / regulation of sensory perception of pain / nitric-oxide synthase (NADPH) / positive regulation of guanylate cyclase activity / cellular response to epinephrine stimulus / nitric oxide mediated signal transduction / xenobiotic catabolic process / sarcoplasmic reticulum membrane / vesicle membrane / nitric-oxide synthase activity / T-tubule / negative regulation of peptidyl-serine phosphorylation / arginine catabolic process / NADPH binding / negative regulation of blood pressure / response to hormone / photoreceptor inner segment / response to organonitrogen compound / nitric oxide biosynthetic process / sarcoplasmic reticulum / positive regulation of histone acetylation / response to activity / muscle contraction / cell periphery / response to nicotine / female pregnancy / response to peptide hormone / positive regulation of long-term synaptic potentiation / response to nutrient levels / secretory granule / sarcolemma / response to organic cyclic compound / response to lead ion / cellular response to growth factor stimulus / cellular response to mechanical stimulus / response to estrogen / Z disc / vasodilation / phosphoprotein binding / establishment of protein localization / positive regulation of neuron death / calcium-dependent protein binding / FMN binding / scaffold protein binding / brain development / flavin adenine dinucleotide binding / NADP binding / transmembrane transporter binding / response to heat / response to ethanol / nuclear membrane / positive regulation of peptidyl-serine phosphorylation / ATPase binding / response to lipopolysaccharide / aging / calmodulin binding / negative regulation of neuron apoptotic process / mitochondrial outer membrane / dendritic spine / response to hypoxia / postsynaptic density / cytoskeleton / oxidoreductase activity / negative regulation of cell population proliferation / glutamatergic synapse / membrane raft / synapse / dendrite
Similarity search - Function
PDZ domain / Pdz3 Domain / Nitric-oxide synthase, eukaryote / Nitric oxide synthase, domain 1 superfamily / Nitric oxide synthase, domain 3 superfamily / Nitric oxide synthase, domain 2 superfamily / Nitric oxide synthase, N-terminal / Nitric oxide synthase (NOS) signature. / Nitric oxide synthase, oxygenase domain / Nitric oxide synthase, N-terminal domain superfamily ...PDZ domain / Pdz3 Domain / Nitric-oxide synthase, eukaryote / Nitric oxide synthase, domain 1 superfamily / Nitric oxide synthase, domain 3 superfamily / Nitric oxide synthase, domain 2 superfamily / Nitric oxide synthase, N-terminal / Nitric oxide synthase (NOS) signature. / Nitric oxide synthase, oxygenase domain / Nitric oxide synthase, N-terminal domain superfamily / FAD binding domain / Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding / NADPH-cytochrome p450 reductase, FAD-binding, alpha-helical domain superfamily / Flavodoxin-like / Flavoprotein pyridine nucleotide cytochrome reductase / Flavodoxin / Oxidoreductase NAD-binding domain / Oxidoreductase FAD/NAD(P)-binding / Flavodoxin-like domain profile. / Flavodoxin/nitric oxide synthase / Ferredoxin-NADP reductase (FNR), nucleotide-binding domain / Ferredoxin reductase-type FAD binding domain profile. / FAD-binding domain, ferredoxin reductase-type / Riboflavin synthase-like beta-barrel / PDZ domain / Flavoprotein-like superfamily / PDZ domain profile. / Domain present in PSD-95, Dlg, and ZO-1/2. / PDZ domain / PDZ superfamily / Roll / Mainly Beta
Similarity search - Domain/homology
Nitric oxide synthase, brain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
MethodSOLUTION NMR
AuthorsTochio, H. / Zhang, Q. / Mandal, P. / Li, M. / Zhang, M.
CitationJournal: Nat.Struct.Biol. / Year: 1999
Title: Solution structure of the extended neuronal nitric oxide synthase PDZ domain complexed with an associated peptide.
Authors: Tochio, H. / Zhang, Q. / Mandal, P. / Li, M. / Zhang, M.
History
DepositionFeb 1, 1999Deposition site: PDBE / Processing site: RCSB
Revision 1.0Apr 29, 1999Provider: repository / Type: Initial release
Revision 1.1Apr 26, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 16, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly ...database_2 / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PROTEIN (NEURONAL NITRIC OXIDE SYNTHASE)
B: PROTEIN (HEPTAPEPTIDE)


Theoretical massNumber of molelcules
Total (without water)14,2972
Polymers14,2972
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / -
Representative

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Components

#1: Protein PROTEIN (NEURONAL NITRIC OXIDE SYNTHASE)


Mass: 13550.674 Da / Num. of mol.: 1 / Fragment: PDZ DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Production host: Escherichia coli (E. coli) / References: UniProt: P29476
#2: Protein/peptide PROTEIN (HEPTAPEPTIDE)


Mass: 745.884 Da / Num. of mol.: 1 / Source method: obtained synthetically

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Sample conditionspH: 6.0 / Temperature: 308 K
Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 15

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