- PDB-2gui: Structure and Function of Cyclized Versions of the Proofreading E... -
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基本情報
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データベース: PDB / ID: 2gui
タイトル
Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. coli DNA Polymerase III
要素
DNA polymerase III epsilon subunit
キーワード
TRANSFERASE / DNA polymerase proofreading domain
機能・相同性
機能・相同性情報
DNA polymerase III, core complex / DNA polymerase III complex / lagging strand elongation / replisome / exonuclease activity / DNA replication proofreading / leading strand elongation / 3'-5' exonuclease activity / DNA-templated DNA replication / DNA-directed DNA polymerase ...DNA polymerase III, core complex / DNA polymerase III complex / lagging strand elongation / replisome / exonuclease activity / DNA replication proofreading / leading strand elongation / 3'-5' exonuclease activity / DNA-templated DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA binding / metal ion binding / cytosol 類似検索 - 分子機能
DNA polymerase 3, epsilon subunit / DNA polymerase III epsilon subunit, exonuclease domain / Exonuclease / Exonuclease, RNase T/DNA polymerase III / EXOIII / Ribonuclease H-like superfamily/Ribonuclease H / Nucleotidyltransferase; domain 5 / Ribonuclease H superfamily / Ribonuclease H-like superfamily / 2-Layer Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
: / DI(HYDROXYETHYL)ETHER / URIDINE-5'-MONOPHOSPHATE / DNA polymerase III subunit epsilon 類似検索 - 構成要素
SEQUENCE The protein is a cyclized form of N-terminal domain of epsilon (residue 2-186). The ...SEQUENCE The protein is a cyclized form of N-terminal domain of epsilon (residue 2-186). The cyclized protein includes 9 amino acid linker, SIEF at the N-terminal and TRESG at the C-terminus
解像度: 1.6→26.88 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.939 / SU B: 1.244 / SU ML: 0.046 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.084 / ESU R Free: 0.085 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.19746
1366
5 %
RANDOM
Rwork
0.16016
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all
0.16195
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obs
0.16195
25755
99.83 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK