Pilus retraction protein PilT/PilU / : / Type II/IV secretion system protein / Type II/IV secretion system protein / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.51 Å3/Da / Density % sol: 50.96 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.6 Details: Upon set up, protein droplet contains 7.5 mg/ml protein in 9.7% PEG1000, 13.0 mM TCEP, 13.0 mM LiCl, 0.32 M AmSO4, 2.6 mM ADP, 16 mM Tris pH 7.6, 98 mM KCl, 130 mM Imidazole, 6.5% glycerol. ...Details: Upon set up, protein droplet contains 7.5 mg/ml protein in 9.7% PEG1000, 13.0 mM TCEP, 13.0 mM LiCl, 0.32 M AmSO4, 2.6 mM ADP, 16 mM Tris pH 7.6, 98 mM KCl, 130 mM Imidazole, 6.5% glycerol. Well contains 0.2 M AmSO4, 10% PEG 1000, unbuffered., VAPOR DIFFUSION, HANGING DROP, temperature 293K
Type: MAR CCD 165 mm / Detector: CCD / Date: Aug 2, 2004
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.97896 Å / Relative weight: 1
Reflection
Redundancy: 3 % / Av σ(I) over netI: 9.1 / Number: 106618 / Rmerge(I) obs: 0.078 / Χ2: 1.05 / D res high: 4.1 Å / D res low: 50 Å / Num. obs: 35971 / % possible obs: 95.7
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