- PDB-2gpq: Cap-free structure of eIF4E suggests basis for its allosteric reg... -
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Basic information
Entry
Database: PDB / ID: 2gpq
Title
Cap-free structure of eIF4E suggests basis for its allosteric regulation
Components
Eukaryotic translation initiation factor 4E
Keywords
TRANSLATION / translation regulation / eIF4E / apo form
Function / homology
Function and homology information
Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / eukaryotic initiation factor 4G binding / regulation of translation at postsynapse, modulating synaptic transmission / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / RNA cap binding / chromatoid body / eukaryotic translation initiation factor 4F complex / Deadenylation of mRNA / mRNA cap binding / Transport of the SLBP independent Mature mRNA ...Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S / eukaryotic initiation factor 4G binding / regulation of translation at postsynapse, modulating synaptic transmission / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / RNA cap binding / chromatoid body / eukaryotic translation initiation factor 4F complex / Deadenylation of mRNA / mRNA cap binding / Transport of the SLBP independent Mature mRNA / M-decay: degradation of maternal mRNAs by maternally stored factors / Transport of the SLBP Dependant Mature mRNA / RNA 7-methylguanosine cap binding / Transport of Mature mRNA Derived from an Intronless Transcript / RISC complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / stem cell population maintenance / mTORC1-mediated signalling / L13a-mediated translational silencing of Ceruloplasmin expression / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of neuron differentiation / behavioral fear response / mRNA export from nucleus / translational initiation / translation initiation factor activity / positive regulation of mitotic cell cycle / cellular response to dexamethasone stimulus / P-body / neuron differentiation / ISG15 antiviral mechanism / cytoplasmic ribonucleoprotein granule / cytoplasmic stress granule / G1/S transition of mitotic cell cycle / regulation of translation / postsynapse / DNA-binding transcription factor binding / negative regulation of translation / nuclear speck / glutamatergic synapse / perinuclear region of cytoplasm / enzyme binding / RNA binding / extracellular exosome / nucleus / cytoplasm / cytosol Similarity search - Function
Ionic strength: 100 mM NaCl / pH: 7.4 / Pressure: ambient / Temperature: 293 K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Varian INOVA
Varian
INOVA
800
2
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Processing
NMR software
Name
Version
Developer
Classification
VNMR
6.1C
Varian
collection
NMRPipe
2.3
Delaglio
processing
Sparky
3.112
Goddard & Kneller
dataanalysis
CNS
1.1
Brunger
refinement
Refinement
Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 1973 restraints, 1697 are NOE-derived distance constraints, 276 dihedral angle restraints, 160 distance restraints from hydrogen bonds
NMR representative
Selection criteria: closest to the average
NMR ensemble
Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 10
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