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Open data
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Basic information
Entry | Database: PDB / ID: 2g8x | ||||||
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Title | Escherichia coli Y209W apoprotein | ||||||
![]() | thymidylate synthase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lee, T.T. / Finer-Moore, J.S. / Stroud, R.M. | ||||||
![]() | ![]() Title: The role of protein dynamics in thymidylate synthase catalysis: Variants of conserved dUMP-binding Tyr-261 Authors: Newby, Z. / Lee, T.T. / Morse, R.J. / Liu, L. / Liu, Y. / Venkatraman, P. / Santi, D.V. / Finer-Moore, J.S. / Stroud, R.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 126.2 KB | Display | ![]() |
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PDB format | ![]() | 97.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1tjsS ![]() 2g8o S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | Chains A and B constitute the biological homodimer |
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Components
#1: Protein | ![]() Mass: 30582.697 Da / Num. of mol.: 2 / Mutation: Y209W, N-terminus is carbamylated Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ![]() #4: Chemical | ![]() #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.66 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 7.8mg/ml protein, 25mM KPO4, pH 7.5, and 5mM DTT equilibrated against a well buffer containing 28% PEG 4K, 100 mM Tris-Cl, pH 8.9, 200mM sodium acetate and 5mM DTT, VAPOR DIFFUSION, HANGING ...Details: 7.8mg/ml protein, 25mM KPO4, pH 7.5, and 5mM DTT equilibrated against a well buffer containing 28% PEG 4K, 100 mM Tris-Cl, pH 8.9, 200mM sodium acetate and 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 173 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 2001 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.83→30.7 Å / Num. all: 41273 / Num. obs: 41273 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 7.3 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 28.1 |
Reflection shell | Resolution: 1.83→1.86 Å / Redundancy: 6 % / Rmerge(I) obs: 0.45 / Mean I/σ(I) obs: 3.2 / Num. unique all: 2073 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB code 1TJS Resolution: 1.83→30.7 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 2091436.2 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: His-51 and Cys-146 in chain A are in two conformations in the crystal
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 57.5412 Å2 / ksol: 0.425534 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.83→30.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.83→1.91 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file |
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