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- PDB-2g8i: B. halodurans RNase H catalytic domain D192N mutant in complex wi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2g8i | ||||||
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Title | B. halodurans RNase H catalytic domain D192N mutant in complex with Mn2+ and RNA/DNA hybrid (non-P nick at the active site) | ||||||
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![]() | HYDROLASE/RNA/DNA / RNase H / Ribonuclease H / RNA/DNA hybrid / HYDROLASE-RNA-DNA COMPLEX | ||||||
Function / homology | ![]() ribonuclease H / RNA-DNA hybrid ribonuclease activity / nucleic acid binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Nowotny, M. / Yang, W. | ||||||
![]() | ![]() Title: Stepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release. Authors: Nowotny, M. / Yang, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63 KB | Display | ![]() |
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PDB format | ![]() | 39.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 465.4 KB | Display | ![]() |
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Full document | ![]() | 466.3 KB | Display | |
Data in XML | ![]() | 12.1 KB | Display | |
Data in CIF | ![]() | 18.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2g8fC ![]() 2g8hC ![]() 2g8kC ![]() 2g8uC ![]() 2g8vC ![]() 2g8wC ![]() 1zbiS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-RNA chain / DNA chain / Protein , 3 types, 3 molecules BCA
#1: RNA chain | Mass: 1875.189 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: DNA chain | Mass: 1824.228 Da / Num. of mol.: 1 / Source method: obtained synthetically |
#3: Protein | Mass: 16329.476 Da / Num. of mol.: 1 / Fragment: Bh-RNase HC / Mutation: D192N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Non-polymers , 4 types, 337 molecules ![](data/chem/img/MN.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-MN / #5: Chemical | ChemComp-CL / | #6: Chemical | ChemComp-MPD / ( | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.09 % | ||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 278 K / Method: vapor diffusion, sitting drop Details: 35% MPD, 0.1M Tris pH 7.0, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 278K | ||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Mar 30, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.65→30 Å / Num. all: 22523 / Num. obs: 20416 / % possible obs: 90.6 % / Observed criterion σ(I): 2.6 |
Reflection shell | Resolution: 1.65→1.71 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ZBI Resolution: 1.65→30 Å / σ(I): 2.6 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.65→30 Å
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Refine LS restraints |
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