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Structure paper

TitleStepwise analyses of metal ions in RNase H catalysis from substrate destabilization to product release.
Journal, issue, pagesEmbo J., Vol. 25, Page 1924-1933, Year 2006
Publish dateMar 2, 2006 (structure data deposition date)
AuthorsNowotny, M. / Yang, W.
External linksEmbo J. / PubMed:16601679
MethodsX-ray diffraction
Resolution1.65 - 2.7 Å
Structure data

PDB-2g8f:
B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-2g8h:
B. halodurans RNase H catalytic domain D192N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-2g8i:
B. halodurans RNase H catalytic domain D192N mutant in complex with Mn2+ and RNA/DNA hybrid (non-P nick at the active site)
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-2g8k:
B. halodurans RNase H catalytic domain D192N mutant in complex with Ca2+ and RNA/DNA hybrid (non-P nick at the active site)
Method: X-RAY DIFFRACTION / Resolution: 1.65 Å

PDB-2g8u:
B. halodurans RNase H catalytic domain D132N mutant in complex with Mg2+ and RNA/DNA hybrid (non-P nick at the active site)
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-2g8v:
B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (reaction product)
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-2g8w:
B. halodurans RNase H catalytic domain E188A mutant in complex with Ca2+ and RNA/DNA hybrid
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-VO4:
VANADATE ION

ChemComp-MN:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-CA:
Unknown entry

ChemComp-EOH:
ETHANOL

Source
  • bacillus halodurans (bacteria)
KeywordsHYDROLASE/RNA/DNA / RNAse H / Ribonuclease H RNA/DNA hybrid / HYDROLASE-RNA-DNA COMPLEX / Ribonuclease H / RNA/DNA hybrid

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