+Open data
-Basic information
Entry | Database: PDB / ID: 2g0z | |||||||||
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Title | Photolyzed CO L29F Myoglobin: 1ns | |||||||||
Components | Myoglobin | |||||||||
Keywords | TRANSPORT PROTEIN / Time-resolved crystallography / myoglobin / difference refinement / structure-function relationship / intermediate states | |||||||||
Function / homology | Function and homology information Oxidoreductases; Acting on other nitrogenous compounds as donors / nitrite reductase activity / sarcoplasm / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / removal of superoxide radicals / oxygen carrier activity / peroxidase activity / oxygen binding / heme binding / extracellular exosome / metal ion binding Similarity search - Function | |||||||||
Biological species | Physeter catodon (sperm whale) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | |||||||||
Authors | Aranda, R. / Levin, E.J. / Schotte, F. / Anfinrud, P.A. / Phillips Jr., G.N. | |||||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 2006 Title: Time-dependent atomic coordinates for the dissociation of carbon monoxide from myoglobin. Authors: Aranda, R. / Levin, E.J. / Schotte, F. / Anfinrud, P.A. / Phillips Jr., G.N. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2g0z.cif.gz | 81.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2g0z.ent.gz | 61.4 KB | Display | PDB format |
PDBx/mmJSON format | 2g0z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2g0z_validation.pdf.gz | 812.3 KB | Display | wwPDB validaton report |
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Full document | 2g0z_full_validation.pdf.gz | 815.4 KB | Display | |
Data in XML | 2g0z_validation.xml.gz | 8.1 KB | Display | |
Data in CIF | 2g0z_validation.cif.gz | 12.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g0/2g0z ftp://data.pdbj.org/pub/pdb/validation_reports/g0/2g0z | HTTPS FTP |
-Related structure data
Related structure data | 2g0rC 2g0sC 2g0vC 2g0xC 2g10C 2g11C 2g12C 2g14C 2splS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Number of models | 2 |
-Components
#1: Protein | Mass: 17399.180 Da / Num. of mol.: 1 / Mutation: L29F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Physeter catodon (sperm whale) / Gene: MB / Production host: Escherichia coli (E. coli) / References: UniProt: P02185 |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HEM / |
#4: Chemical | ChemComp-CMO / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.13 % |
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Crystal grow | Temperature: 298 K / pH: 9 Details: 2.6M Ammonium Sulfate, 20mM TrisHCl, 1mM EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 9.00 |
-Data collection
Diffraction | Mean temperature: 283 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID09 / Wavelength: 0.79 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 1, 2002 |
Radiation | Protocol: LAUE / Monochromatic (M) / Laue (L): L / Scattering type: neutron |
Radiation wavelength | Wavelength: 0.79 Å / Relative weight: 1 |
Reflection | Resolution: 1.801→29.881 Å / Num. obs: 18341 / Biso Wilson estimate: 15.8 Å2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY: 2SPL Resolution: 1.95→12.7 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 1535611.03 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: DIFFERENCE REFINEMENT (TERWILLIGER 1995
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Displacement parameters | Biso mean: 20.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.95→12.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.07 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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