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Open data
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Basic information
Entry | Database: PDB / ID: 2g10 | ||||||
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Title | Photolyzed CO L29F Myoglobin: 3.16ns | ||||||
![]() | Myoglobin | ||||||
![]() | TRANSPORT PROTEIN / Time-resolved crystallography / myoglobin / difference refinement / structure-function relationship / intermediate states | ||||||
Function / homology | ![]() Oxidoreductases; Acting on other nitrogenous compounds as donors / nitrite reductase activity / sarcoplasm / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / removal of superoxide radicals / oxygen carrier activity / peroxidase activity / oxygen binding / heme binding / extracellular exosome / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Aranda, R. / Levin, E.J. / Schotte, F. / Anfinrud, P.A. / Phillips Jr., G.N. | ||||||
![]() | ![]() Title: Time-dependent atomic coordinates for the dissociation of carbon monoxide from myoglobin. Authors: Aranda, R. / Levin, E.J. / Schotte, F. / Anfinrud, P.A. / Phillips Jr., G.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82 KB | Display | ![]() |
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PDB format | ![]() | 61.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2g0rC ![]() 2g0sC ![]() 2g0vC ![]() 2g0xC ![]() 2g0zC ![]() 2g11C ![]() 2g12C ![]() 2g14C ![]() 2splS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Number of models | 2 |
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Components
#1: Protein | Mass: 17399.180 Da / Num. of mol.: 1 / Mutation: L30F,D123N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-HEM / |
#4: Chemical | ChemComp-CMO / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.15 Å3/Da / Density % sol: 60.99 % |
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Crystal grow | Temperature: 298 K / pH: 9 Details: 2.6M Ammonium Sulfate, 20mM TrisHCl, 1mM EDTA, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 9.00 |
-Data collection
Diffraction | Mean temperature: 283 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 1, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.79 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→29.881 Å / Num. obs: 22202 / Biso Wilson estimate: 19 Å2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY: 2SPL Resolution: 1.9→29.88 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 1659249.64 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: DIFFERENCE REFINEMENT (TERWILLIGER 1995
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Displacement parameters | Biso mean: 24 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→29.88 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.02 Å / Rfactor Rfree error: 0.008 / Total num. of bins used: 6
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Xplor file |
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