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Yorodumi- PDB-2fz0: Identification of yeast R-SNARE Nyv1p as a novel longin domain protein -
+Open data
-Basic information
Entry | Database: PDB / ID: 2fz0 | ||||||
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Title | Identification of yeast R-SNARE Nyv1p as a novel longin domain protein | ||||||
Components | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane; Nyv1pSNARE (protein) | ||||||
Keywords | MEMBRANE PROTEIN / SNARE protein / Longin domain | ||||||
Function / homology | Function and homology information trans-Golgi Network Vesicle Budding / vacuole fusion, non-autophagic / vesicle fusion / SNARE complex / SNAP receptor activity / fungal-type vacuole membrane / vesicle-mediated transport / membrane => GO:0016020 Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Wen, W. / Zhang, M. | ||||||
Citation | Journal: Mol.Cell.Biol. / Year: 2006 Title: Identification of the Yeast R-SNARE Nyv1p as a Novel Longin Domain-containing Protein Authors: Wen, W. / Chen, L. / Wu, H. / Sun, X. / Zhang, M. / Banfield, D.K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fz0.cif.gz | 918.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fz0.ent.gz | 792.2 KB | Display | PDB format |
PDBx/mmJSON format | 2fz0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fz/2fz0 ftp://data.pdbj.org/pub/pdb/validation_reports/fz/2fz0 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17014.383 Da / Num. of mol.: 1 / Fragment: longin domain, Residues 1-149 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Plasmid: pETH / Production host: Escherichia coli (E. coli) / References: UniProt: Q12255 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | pH: 7.0 / Pressure: ambient / Temperature: 308 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: CNS / Version: 1.1 / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |