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- PDB-2fuj: A putative acyl-CoA thioesterase from Xanthomonas campestris (XC229) -
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Open data
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Basic information
Entry | Database: PDB / ID: 2fuj | ||||||
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Title | A putative acyl-CoA thioesterase from Xanthomonas campestris (XC229) | ||||||
![]() | conserved hypothetical protein | ||||||
![]() | HYDROLASE / Xanthomonas campestris / structural genomics / conserved hypothetical protein / hot dog domain / acyl-CoA thioesterase | ||||||
Function / homology | fatty acyl-CoA hydrolase activity / Hotdog Thioesterase / Thiol Ester Dehydrase; Chain A / HotDog domain superfamily / Roll / Alpha Beta / Uncharacterized protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chin, K.H. / Chou, C.C. / Wang, A.H. / Chou, S.H. | ||||||
![]() | ![]() Title: Crystal structure of a putative acyl-CoA thioesterase from Xanthomonas campestris (XC229) adopts a tetrameric hotdog fold of epsilongamma mode. Authors: Chin, K.H. / Chou, C.C. / Wang, A.H. / Chou, S.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 39.7 KB | Display | ![]() |
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PDB format | ![]() | 28 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 364.4 KB | Display | ![]() |
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Full document | ![]() | 371.3 KB | Display | |
Data in XML | ![]() | 4.9 KB | Display | |
Data in CIF | ![]() | 7.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | XC229 was shown to be a homotetramer. However, there was only one subunit per asymmetric unit in the crystals employed in this study. The tetramer was constructed by rotating the contents of the asymmetric unit using the crystallographic I-centered 2-fold symmetry(I23). |
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Components
#1: Protein | Mass: 15198.329 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Species: Xanthomonas campestris / Strain: ATCC 33913 / Plasmid: pET20b / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.32 Å3/Da / Density % sol: 62.94 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8 Details: MPD33%, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 3, 2005 |
Radiation | Monochromator: Se-Met / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9823 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→26.6 Å / Num. all: 43207 / Num. obs: 41911 / % possible obs: 97.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 1.7→26.6 Å
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