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Yorodumi- PDB-2fgy: Beta Carbonic Anhydrase from the Carboxysomal Shell of Halothioba... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2fgy | ||||||
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Title | Beta Carbonic Anhydrase from the Carboxysomal Shell of Halothiobacillus neapolitanus (CsoSCA) | ||||||
Components | carboxysome shell polypeptide | ||||||
Keywords | LYASE / beta class of carbonic anhydrase | ||||||
Function / homology | Function and homology information carboxysome / carbon fixation / carbonic anhydrase / carbonate dehydratase activity / metal ion binding Similarity search - Function | ||||||
Biological species | Halothiobacillus neapolitanus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIRAS / Resolution: 2.2 Å | ||||||
Authors | Sawaya, M.R. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2006 Title: The Structure of beta-carbonic anhydrase from the carboxysomal shell reveals a distinct subclass with one active site for the price of two. Authors: Sawaya, M.R. / Cannon, G.C. / Heinhorst, S. / Tanaka, S. / Williams, E.B. / Yeates, T.O. / Kerfeld, C.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fgy.cif.gz | 204.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fgy.ent.gz | 167 KB | Display | PDB format |
PDBx/mmJSON format | 2fgy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fg/2fgy ftp://data.pdbj.org/pub/pdb/validation_reports/fg/2fgy | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Refine code: 1
NCS ensembles :
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Details | The biological assembly is a dimer. It is contained in the asymmetric unit. |
-Components
#1: Protein | Mass: 60718.145 Da / Num. of mol.: 2 / Mutation: Y92H Source method: isolated from a genetically manipulated source Source: (gene. exp.) Halothiobacillus neapolitanus (bacteria) Description: Gene name is previously known as CsoS3 / Gene: CsoSCA / Plasmid: pcsoS3ProEx / Production host: Escherichia coli (E. coli) References: GenBank: 3449369, UniProt: O85042*PLUS, carbonic anhydrase #2: Chemical | ChemComp-ZN / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.57 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.1M HEPES, pH 7.0, 0.2M magnesium chloride, 10% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1.0332 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 8, 2005 / Details: KOHZU: Double Crystal Si(111) |
Radiation | Monochromator: Double Crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→90 Å / Num. all: 52342 / Num. obs: 52789 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 5.7 % / Biso Wilson estimate: 44.1 Å2 / Rmerge(I) obs: 0.123 / Χ2: 1.089 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.488 / Num. unique all: 5120 / Χ2: 1.215 / % possible all: 94.7 |
-Phasing
Phasing | Method: MIRAS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Phasing MAD | D res high: 2.2 Å / D res low: 75.38 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Phasing dm | Method: Solvent flattening and Histogram matching / Reflection: 52789 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Phasing dm shell |
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Phasing MIR der | R cullis centric: 0 / Der set-ID: 1 / Loc centric: 0 / Power centric: 0 / Reflection centric: 0
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Phasing MIR der shell | R cullis centric: 0 / Loc centric: 0 / Power centric: 0 / Reflection centric: 0
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