+Open data
-Basic information
Entry | Database: PDB / ID: 2f2n | ||||||
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Title | Triclinic hen egg lysozyme cross-linked by glutaraldehyde | ||||||
Components | Lysozyme C | ||||||
Keywords | HYDROLASE / denaturation / lysozyme / barnase / cross-linked-crystals / urea / thiourea / bromoethanol | ||||||
Function / homology | Function and homology information Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium ...Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 1.603 Å | ||||||
Authors | Prange, T. / Salem, M. / Mauguen, Y. | ||||||
Citation | Journal: Biochim.Biophys.Acta / Year: 2006 Title: On the edge of the denaturation process: Application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations. Authors: Salem, M. / Mauguen, Y. / Prange, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2f2n.cif.gz | 43 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2f2n.ent.gz | 27.7 KB | Display | PDB format |
PDBx/mmJSON format | 2f2n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f2/2f2n ftp://data.pdbj.org/pub/pdb/validation_reports/f2/2f2n | HTTPS FTP |
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-Related structure data
Related structure data | 2f30C 2f4aC 2f4gC 2f4yC 2f56C 2f5mC 2f5wC 1lksS 3lztS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: eggs / Source: (natural) Gallus gallus (chicken) / References: UniProt: P00698, lysozyme | ||
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#2: Chemical | ChemComp-NO3 / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.81 Å3/Da / Density % sol: 32.01 % |
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Crystal grow | Temperature: 300 K / Method: vapor diffusion, hanging drop / pH: 6 Details: sodium nitrate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: SYNCHROTRON / Site: LURE / Beamline: DW32 / Wavelength: 0.974 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jan 1, 2000 |
Radiation | Monochromator: SI(III) CURVATED MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.974 Å / Relative weight: 1 |
Reflection | Resolution: 1.603→8.7 Å / Num. all: 12253 / Num. obs: 12315 / % possible obs: 93.55 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 4 / Redundancy: 4.3 % / Biso Wilson estimate: 12.3 Å2 / Rmerge(I) obs: 0.045 / Rsym value: 0.043 / Net I/σ(I): 32.01 |
Reflection shell | Resolution: 1.603→1.7 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.174 / Mean I/σ(I) obs: 17.2 / Rsym value: 0.168 / % possible all: 87.84 |
-Processing
Software |
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Refinement | Method to determine structure: FOURIER SYNTHESIS Starting model: PDB ENTRY 3LZT, 1LKS Resolution: 1.603→8 Å / Num. parameters: 4727 / Num. restraintsaints: 4203 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 954 / Occupancy sum non hydrogen: 1179 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.603→8 Å
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Refine LS restraints |
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LS refinement shell |
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