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- PDB-2erb: AgamOBP1, and odorant binding protein from Anopheles gambiae comp... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2erb | ||||||
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Title | AgamOBP1, and odorant binding protein from Anopheles gambiae complexed with PEG | ||||||
![]() | odorant binding protein | ||||||
![]() | TRANSPORT PROTEIN / helix / disulfides | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wogulis, M. / Morgan, T. / Ishida, Y. / Leal, W.S. / Wilson, D.K. | ||||||
![]() | ![]() Title: The crystal structure of an odorant binding protein from Anopheles gambiae: Evidence for a common ligand release mechanism. Authors: Wogulis, M. / Morgan, T. / Ishida, Y. / Leal, W.S. / Wilson, D.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 77.4 KB | Display | ![]() |
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PDB format | ![]() | 55.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Details | The biological assembly is likely a monomer. |
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Components
#1: Protein | Mass: 14547.619 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: pET-22b(+) / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Chemical | ChemComp-MG / | #3: Chemical | ChemComp-PEU / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.28 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 32% PEG 8000, 250 mM MgCl2, 50 mM Tris HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 8, 2005 |
Radiation | Monochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.88557 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→30 Å / Num. all: 39215 / Num. obs: 39190 / % possible obs: 99.9 % / Redundancy: 3.8 % / Biso Wilson estimate: 14.2 Å2 / Rmerge(I) obs: 0.038 |
Reflection shell | Resolution: 1.5→1.54 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.133 / Mean I/σ(I) obs: 3.1 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Monomer of model generated from MAD experiments. Resolution: 1.5→30.3 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 121865.73 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 41.145 Å2 / ksol: 0.380672 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.5→30.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.5→1.59 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6
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Xplor file |
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