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Yorodumi- PDB-2enj: Solution structure of the C2 domain from human protein kinase C theta -
+Open data
-Basic information
Entry | Database: PDB / ID: 2enj | ||||||
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Title | Solution structure of the C2 domain from human protein kinase C theta | ||||||
Components | Protein kinase C theta type | ||||||
Keywords | TRANSFERASE / beta-sandwich / phosphotyrosine binding / TCR / T-cell / diacylglycerol / phorbol ester / PKC / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information positive regulation of T-helper 2 cell activation / protein kinase C / Netrin-1 signaling / diacylglycerol-dependent serine/threonine kinase activity / Effects of PIP2 hydrolysis / regulation of platelet aggregation / CD4-positive, alpha-beta T cell proliferation / positive regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of T cell apoptotic process / positive regulation of T-helper 17 type immune response ...positive regulation of T-helper 2 cell activation / protein kinase C / Netrin-1 signaling / diacylglycerol-dependent serine/threonine kinase activity / Effects of PIP2 hydrolysis / regulation of platelet aggregation / CD4-positive, alpha-beta T cell proliferation / positive regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of T cell apoptotic process / positive regulation of T-helper 17 type immune response / aggresome / positive regulation of interleukin-4 production / Apoptotic cleavage of cellular proteins / positive regulation of interleukin-17 production / : / positive regulation of telomere capping / Fc-epsilon receptor signaling pathway / membrane protein ectodomain proteolysis / immunological synapse / centriolar satellite / negative regulation of insulin receptor signaling pathway / positive regulation of telomere maintenance via telomerase / positive regulation of interleukin-2 production / cell chemotaxis / axon guidance / regulation of cell growth / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / FCERI mediated NF-kB activation / G alpha (z) signalling events / RAS processing / Inactivation, recovery and regulation of the phototransduction cascade / positive regulation of T cell activation / Downstream TCR signaling / positive regulation of NF-kappaB transcription factor activity / protein kinase activity / intracellular signal transduction / inflammatory response / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / regulation of DNA-templated transcription / ATP binding / metal ion binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Nagashima, T. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the C2 domain from human protein kinase C theta Authors: Nagashima, T. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2enj.cif.gz | 825.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2enj.ent.gz | 692.7 KB | Display | PDB format |
PDBx/mmJSON format | 2enj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2enj_validation.pdf.gz | 342.3 KB | Display | wwPDB validaton report |
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Full document | 2enj_full_validation.pdf.gz | 464.3 KB | Display | |
Data in XML | 2enj_validation.xml.gz | 39.3 KB | Display | |
Data in CIF | 2enj_validation.cif.gz | 63.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/en/2enj ftp://data.pdbj.org/pub/pdb/validation_reports/en/2enj | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15374.555 Da / Num. of mol.: 1 / Fragment: C2 domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Plasmid: P051205-03 / References: UniProt: Q04759, protein kinase C |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.28mM uniformly 13C, 15N-labeled protein; 20mM TrisHCl, 100mM NaCl, 1mM DTT, 0.02%NaN3, 10% D2O, 90% H2O Solvent system: 10% D2O / 90% H2O |
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Sample conditions | Ionic strength: 120mM / pH: 7 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |