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Yorodumi- PDB-2elg: The rare crystallographic structure of d(CGCGCG)2: The natural sp... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2elg | ||||||||||||||||||
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Title | The rare crystallographic structure of d(CGCGCG)2: The natural spermidine molecule bound to the minor groove of left-handed Z-DNA d(CGCGCG)2 at 10 degree celsius | ||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / d(CGCGCG)2 / left-handed Z-DNA / spermidine / polyamine | Function / homology | SPERMIDINE / DNA | Function and homology information Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1 Å | Authors | Ohishi, H. / Tozuka, Y. / Zhou, D.Y. / Ishida, T. / Nakatani, K. | Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2007 | Title: The rare crystallographic structure of d(CGCGCG)(2): The natural spermidine molecule bound to the minor groove of left-handed Z-DNA d(CGCGCG)(2) at 10 degrees C Authors: Ohishi, H. / Tozuka, Y. / Da-Yang, Z. / Ishida, T. / Nakatani, K. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2elg.cif.gz | 22.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2elg.ent.gz | 14.8 KB | Display | PDB format |
PDBx/mmJSON format | 2elg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/el/2elg ftp://data.pdbj.org/pub/pdb/validation_reports/el/2elg | HTTPS FTP |
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-Related structure data
Related structure data | 1dj6S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 1810.205 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: This sequence occurs naturally in humans. #2: Chemical | ChemComp-SPD / | #3: Chemical | #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.681153 Å3/Da / Density % sol: 26.83592 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 283 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 10mM Sodium cacodilate, 1M Magnesium chloride, 100mM spermidine trichloride, 8%MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Dec 15, 2005 / Details: mirrors |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1→25 Å / Num. obs: 11287 / % possible obs: 99.5 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 4.7 % / Biso Wilson estimate: 5.3 Å2 / Rmerge(I) obs: 0.047 / Rsym value: 0.057 / Net I/σ(I): 20.7 |
Reflection shell | Highest resolution: 1 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.047 / Mean I/σ(I) obs: 20.7 / Num. unique all: 11287 / Rsym value: 0.057 / % possible all: 99.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1DJ6 Resolution: 1→7 Å / Isotropic thermal model: Isotropic / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 5.8 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1→7 Å
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